Food / Water / Environmental

Food, Water & Environmental Bottled & Bagged Media List

Bottled & Bagged ready to use culture media for the food, water and environmental microbiological testing laboratories: Broths, agars and diluents are dispensed into various containers and volumes using both manual and fully automated microbiology laboratory equipment production lines. An extensive range of containers/volumes and microbiological media formulations are on offer to meet your individual requirements.

 

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  • Alkaline Peptone Water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. The high pH of the medium inhibits most enteric organisms for at least 24 hours. The medium is heavily inoculated with faeces and after not more than 8 hours incubation a loopful from the top of the medium is sub cultured onto TCBS Agar. This enrichment medium is also used for food and water testing.
  • Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.
  • A very nutritious isotonic general purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture.
  • This medium is used to detect and/or confirm the presence of coli-aerogenes group of organisms in water, food and dairy laboratories. Bile and Brilliant Green are included in the medium to inhibit gram positive organisms while the coli-aerogenes organisms are identified by the formation of gas during the fermentation of Lactose. The medium can also be used for the confirmation of Escherichia coli by incubating at 44°C.
  • This is a modification of the original Nitrate Reduction Broth which is generally used as one of a series of identification tests for the enterobacteriaceae group of organisms. In addition to allowing the testing of Nitrate Reduction this formulation also contains Agar making it possible to concurrently determine motility. The medium is recommended for use in the confirmatory testing of Clostridium perfringens in water samples. The medium is inoculated by “stabbing” the test organism into the medium, using an inoculating needle or straight wire, and after appropriate incubation motility is demonstrated by diffusion of the organism from the line of inoculation into the medium. The Nitrate Reduction Test is a test for the presence of the enzyme nitrate reductase which, in the presence of an appropriate electron donor, reduces nitrate to nitrite. Following incubation “Nitrate Reagent” is added to the medium and a positive reaction is indicated by the formation of a red colour. For full details of the test method reference should be made to appropriate publications.
  • A pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective medium. This non-selective, nutritious medium is free from inhibitors and is well buffered to maintain the pH at 7.0 for the incubation period according to ISO 6579 (2002).
  • This is one of a number of selective enrichment broths that can be used in the isolation of Campylobacter spp from clinical, food and environmental specimens and contains nutrients to aid in the resuscitation of damaged organisms. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Vancomycin, Cefoperazone, Trimethoprim and Amphotericin B. Following incubation at 37ºC the broth is usually sub-cultured onto an appropriate solid Campylobacter medium.
  • The principle use for this product is in the testing of disinfectants and antiseptics. In 1989, the European Committee for Standardisation (CEN) set up a technical committee to produce harmonised test methods for disinfectants and antiseptics. The CEN standards provide a useful basis for disinfectant validation, and although alternative methods could be used for assessing disinfectant efficacy, following the same basic methods allows not only direct comparison between products but also comparison across various different laboratories. The adaptability of the methods - numerous validation studies based on the CEN methods have been accepted by both the European and US regulatory authorities - allows end- users to customise the methods to their specific requirements. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol). L-histidine, in combination with lecithin and Tween 80, neutralises aldehydes and formaldehyde generating agents. Sodium thiosulphate neutralises iodine and chlorine.
  • BM0090

    Columbia Agar

    Columbia Agar is a nutritious general-purpose basal medium capable of supporting the growth of most micro-organisms, including many fastidious organisms that do not require blood. However when further enriched with Sterile Blood, which can be “chocolated” if required, the medium is generally used for the isolation of most clinically significant pathogens.  The medium can be made selective for various groups of organisms by the addition of a range of antimicrobial supplements. This formulation complies with the Harmonized USP/EP/JP.
  • Prepared from Minced Meat granules overlaid with Brain Heart Infusion Broth this medium is suitable for recovery, isolation and storage of the most fastidious anaerobes as well as many aerobes.
  • All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn.
  • All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn.
  • A sterile concentrated emulsion of premium egg yolks, suitable for incorporation in culture media which detect lecithinase production by bacteria. It can be used in media for Bacillus cereus and Staphylococci. Used with serum and Filde’s extract it may be used to produce Nagler plates for Clostridia.
  • This is a sterile emulsion of Egg Yolk in Saline containing Potassium Tellurite and is generally used as a selective differential agent in Baird Parker Medium. The complete medium is selective for Staphylococcus aureus as the Potassium Tellurite inhibits most coliform organisms and is also reduced by Staphylococcus aureus to tellurite giving typical black colonies on the Baird Parker Medium.
  • Enterobacteriaceae Enrichment Broth E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non lactose fermenting organisms.
  • BM0640

    Fraser Broth

    A modification of UVM Medium, Fraser Broth is a secondary selective enrichment broth for the isolation of Listeria spp primarily from food and environmental specimens. The medium is made selective by the inclusion of Nalidixic Acid and Acriflavine. Darkening of the broth following incubation, due to the presence of Aesculin and Ferric Ammonium Citrate, is indicative of the presence of Listeria spp. Lithium Chloride is also included to inhibit the growth of enterococci that would otherwise hydrolyse the Aesculin. This medium is generally used in conjunction with Fraser Broth Half-Strength (BM0647). NB:  It should be noted that the lack of darkening of the broth should not be taken as a final negative result and all Fraser Broth enrichment cultures should be sub-cultured onto an appropriate selective agar medium irrespective of colour.