Chromogenic Pre Poured Petri Dishes

E&O manufacture and stock a comprehensive range of high quality standard and bespoke formulations of readytouse plated culture media in several different petridish sizes and media depths to accommodate all microbial growth requirements. Packaging formats comply with all industry sector regulations.

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  • Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. Colorex™ UTI has been developed primarily for use in the examination of urine specimens with the aim of simplifying the differentiation and presumptive identification of the main organisms, gram negative and gram positive, usually found in Urinary Tract Infections. It can however be used to differentiate organisms in other types of clinical specimens. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium.
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  • Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the Enterobacteriaceae. Colorex™ ESBL (Extended Spectrum Beta-Lactamase) medium has been developed for the isolation of ESBL – producing organisms with the aim of simplifying the differentiation and presumptive identification of the causative organism. It should be noted that other non-ESBL and AmpC isolates will be inhibited on this medium reducing the incidence of false positives. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium. Typical colour reactions are as follows: Escherichia coli – Red colonies; Proteus spp., Providencia spp. & Morganella spp. – Clear colonies with a brown halo; Klebsiella spp., Enterobacter spp, Serratia spp. & Citrobacter spp. – Metallic blue colonies; Salmonella spp. & Acinetobacter spp. – Clear colonies; Gram +ve bacterial species and yeasts – Inhibited.
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  • This is a chromogenic medium based on CLED that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections. Reduced electrolyte concentration prevents swarming of Proteus spp. A sophisticated binary chromogenic system and supplementation with tryptophan allows differentiation of Enterococcus spp. (turquoise colonies), Proteus spp. (clear colonies with a brown halo), Enterobacter spp. (metallic blue colonies), Staphylococcus spp. (white colonies), and E. coli (purple colonies).
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  • Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed with the aim of simplifying the differentiation and presumptive identification of the main organisms usually found in Urinary Tract Infections. Based on the traditional CLED Medium, to prevent the swarming of Proteus spp, two chromogens are present in the medium. One allows the detection of enterococci giving rise to blue colonies whilst the second results in purple colonies of E. coli. Phenylalanine and Tryptophan are also included as indicators of Tryptophan deaminase activity producing brown colonies of Proteus spp. This media is an opaque version to aid differentiation and presumptive identification of the bacteria isolated.
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  • CLED Agar & Primary UTI Chromogenic Agar - Opaque Side One CLED Agar: Mackey and Sandy’s formulation this medium is popular for Urine Culture in the clinical laboratory. The lack of electrolytes inhibits the spreading of Proteus spp. and Bromothymol Blue indicator allows easy differentiation of Lactose and Non-Lactose fermenting organisms. Cystine is also present to benefit those organisms that have a particular Cystine requirement. Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium. Side Two Primary UTI chromogenic agar - opaque: Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed with the aim of simplifying the differentiation and presumptive identification of the main organisms usually found in Urinary Tract Infections. This is an opaque version of the Primary UTI Chromogenic Agar (PP3005). It is based on the traditional CLED Medium to prevent the swarming of Proteus spp. Two chromogens are present in the medium, one allows the detection of enterococci giving rise to blue colonies whilst the second results in purple colonies of E. coli. Phenylalanine and Tryptophan are also included as indicators of Tryptophan Deaminase activity producing brown colonies of Proteus spp. Quality Control Test Organisms: *Organisms - CLED **Organims - Primary UTI
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  • In recent years there has been an increase in the number of immuno-compromised patients, which has in turn led to an increased rate of infections associated with Candida species. There were 2151 reported cases of candidaemia in 2016 with C.albicans accounting for 42%, C.glabrata for 25%, C.parapsilosis for 9% and C.tropicalis for 3% of infections in England, Wales and Northern Ireland.(1) COLOREX™ Candida was formulated specifically for the detection and isolation of clinically significant Candida spp. by means of colonial colour and morphology within 48hrs. COLOREX™ Candida allows for the recognition of a minor Candida population within a mixed population as well as the pre-dominant species thereby allowing for a patient specific treatment plan at the earliest possible opportunity. Most bacterial species will be inhibited due to the inclusion of chloramphenicol. C.albicans – Green colonies C.tropicalis – Metallic blue colonies C.glabrata – Mauve to pink colonies C.krusei – Large fuzzy pink colonies Limitations Definitive identification requires additional testing of isolates (e.g. MALDI-TOF). C.glabrata and C.parapsilosis cannot be readily distinguished on this particular medium. C.dubliniensis will form dark green colonies on COLOREX™ Candida so additional testing is required to confirm presence in the specimen. C.auris isolates will grow on this medium but the colony colour may vary from white to pale purple/pink so further testing will be required to confirm identification.
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  • Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This medium was developed as a selective medium for the isolation and enumeration of Escherichia coli and coliforms in food and water samples. Escherichia coli can be easily differentiated from other coliform organisms by the presence of blue colonies with the other coliforms producing red colonies.
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  • Colorex™ Acinetobacter Colorex™ Acinetobacter is a chromogenic medium for the detection of Acinetobacter spp. Positive colonies exhibit a distinct red colouration with a pale grey centre enabling easy interpretation amongst blue, violet or colourless colonies that may be produced by other Gram –ve bacteria. Gram +ve bacteria and yeast are inhibited on this medium. Some Pseudomonas and Burkholderia spp. may grow on this medium producing pale red colonies but are readily distinguishable due to differences in colonial morphology compared to the Acinetobacter spp.
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