Clinical / Veterinary

Clinical & Veterinary Pre Poured Petri Dishes

E&O manufacture and stock a comprehensive range of high quality standard and bespoke formulations of readytouse plated culture media in several different petridish sizes and media depths to accommodate all microbial growth requirements. Packaging formats comply with all industry sector regulations.

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  • This is a selective medium for the isolation of Actinomyces spp. from clinical specimens. It is based on Fastidious Anaerobe Agar that has been enriched with 7% defibrinated horse blood and made selective by the addition of nalidixic acid to inhibit most aerobes (particularly Gram-negative bacilli) and metronidazole to suppress other anaerobes.
  • This is a 25ml fill selective medium for the isolation of Actinomyces spp from clinical specimens. Based on Fastidious Anaerobe Agar enriched with 7% Horse Blood, the medium has been made selective by the inclusion of Nalidixic Acid to inhibit most aerobes, particularly gram-negative bacilli, and Metronidazole to suppress other anaerobes.
  • Bacillus Cereus (Mannitol Egg Yolk Polymixin - MYP) Agar Intended for the selective enumeration of Bacillus cereus in food samples the medium uses two reactions (Mannitol fermentation and Lecithinase production) to differentiate Bacillus cereus from other members of the species. As Bacillus cereus is Mannitol Negative the colonies are pink in colour due to the presence of Phenol Red Indicator. Lecithinase production (from the Egg Yolk) is indicated by a white precipitate around the colonies. Polymixin is added as the selective agent to inhibit coliforms. NB: It should be noted that some Proteus spp and gram positive cocci may grow on this medium.
  • Bile Aesculin Agar Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.
  • A general-purpose medium enriched with 7% Horse Blood, suitable for the isolation of most organisms including many fastidious anaerobes of clinical significance.
  • Brazier's CCEY Agar with 1% Lysed Horse Blood - Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light.
  • Brucella Agar is a non-selective medium that is enriched with peptones, 5% sheep blood, haemin and vitamin K to support the growth of fastidious, slow growing, obligately anaerobic micro-organisms.
  • Brucella agar is an enriched non-selective medium that supports the growth of fastidious and slow growing, obligately anaerobic micro-organisms due to its content of peptones, dextrose and yeast extract. The medium is supplemented with sheep blood, haemin and vitamin K which provide essential nutrients for certain anaerobes.
  • This is a selective medium for the isolation of Burkholderia cepacia. The base contains Bile Salts and Crystal Violet as selective agents and Ticarcillin and Polymixin B are added as additional supplements to further improve the selectivity particularly inhibition of most Pseudomonas spp.
  • This medium is designed for the selective isolation of Campylobacter spp., particularly C. jejuni and C. coli , which are major causes of gastrointestinal infection. Campylobacter Blood Free Agar CCDA was formulated to allow replacement of blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate. Selectivity is achieved through the addition of broad spectrum cefoperazone and amphotericin B to inhibit fungi. Inoculated plates are incubated at 41.5°C to improve growth of the thermophilic species including C. jejuni and C. coli. Campylobacter Blood Free Agar CCDA is recommended for food testing in compliance with the requirements of ISO 10272- 1:2017(1) . It can also be used for isolation of Campylobacter spp. from clincal specimens such as faeces(2,3). 1. ISO 10272-1:2017. Microbiology of food and animal feeding stuffs – Horizontal method for detection and enumeration of Campylobacter spp. – Part 1: Detection method. 2. Public Health England. (2014). Investigations of Faecal Specimens for Enteric Pathogens. UK Standards for Microbiology Investigations. B 30 Issue 8.1. 3. Public Health England. (2018). Identification of Campylobacter species. UK Standards for Microbiology Investigations. ID 23 Issue 3.1.
  • Campylobacter Blood Free CCDA Agar One of several media formulations available for the selective isolation of Campylobacter spp., primarily C.jejuni and C.coli, which are the leading cause of enteric illness in the UK. Campylobacter spp. can cause mild to severe diarrhoea, usually self-limiting, but some specific serotypes can trigger acute post-infective conditions affecting the peripheral nervous system, such as Guillain-Barré Syndrome. Campylobacter Blood-Free Selective Medium (CCDA) was first described by Bolton. This medium was formulated to replace blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate. Also, in order to improve selectivity, Cefoperazone replaced the Cephazolin utilised in the original formulation. Bolton recommended incubating inoculated plates at 37°C to improve isolation rates but incubation at 41.5°C is recommended for the isolation of the commonly encountered thermophilic species (C.jejuni & C.coli). Yeast and fungal contaminants can be suppressed with the addition of Amphotericin B. Campylobacter Blood-Free Selective Medium (CCDA) is recommended for food testing. Campylobacter Blood-Free Selective Medium with the addition of Yeast Extract and Cefoperazone is used in the Isolation of Campylobacter species from foodstuffs and swabs in the FDA/BAM Method. The product complies with the requirements of ISO 10272-1:2006.
  • Campylobacter Selective (Skirrow) Agar This is one of several selective media available for the isolation of Campylobacter spp in clinical, food and environmental laboratories. Based on Columbia Agar enriched with Lysed Horse Blood. Polymyxin B, Trimethoprim & Vancomycin are added as the selective agents. Sodium Thiosulphate, Pyruvic Acid and Ferrous Sulphate are also included to enhance the aerotolerance of Campylobacter spp. NB: This medium should be incubated at 42°C to optimise selectivity.
  • Cetrimide Agar is intended primarily for use in the isolation of Pseudomonas aeruginosa from pharmaceutical products and is recommended by the United States Pharmacopoeia for this purpose. The medium is made selective by the addition of cetrimide to inhibit the growth of most other organisms while allowing Pseudomonas aeruginosa/ and other Pseudomonas spp. to develop a classical colonial appearance, producing green pigmentation and fluoresce when examined under ultra violet light.
  • This is a medium intended for the cultivation and isolation of Bordetella pertussis & Haemophilus spp. The base medium contains Charcoal and is enriched with 10% Horse Blood. It can also be used as a maintenance or transport medium for these organisms.
  • Charcoal Agar with 10% Horse Blood & Cephalexin This is one of two media generally used for the selective isolation of Bordetella pertussis. The medium is made selective by the inclusion of Cephalexin, to suppress the unwanted naso-pharyngeal flora often present in specimens submitted for the isolation of Bordetella pertussis, and further enriched with 10% Horse Blood. NB: Although coliforms are inhibited by this medium, Pseudomonas aeruginosa and some fungi will grow.
  • A highly nutritious medium used for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophillus species from a variety of clinical specimens. The media is further enriched with Suplex (Polyvitex) that provides vitamins, amino acids, co-enzymes, glucose and other factors which improve the growth of Neisseria and Haemophillus species.