Brain Heart Infusion (BHI) Broth

Brain Heart Infusion (BHI) Broth

A very nutritious isotonic general purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms.

NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture.

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Organisms Ref. No Result
Escherichia coli NCTC 12241 Growth
Staphylococcus aureus NCTC 12981 Growth
Streptococcus pneumoniae NCTC 12977 Growth