BM0440 – Triple Sugar Iron Agar (Slope)

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BM0440 – Triple Sugar Iron Agar (Slope)

This is a medium that can be used to differentiate between some of the Enterobacteriacae on the basis of four reactions, fermentation of Lactose, Glucose and Sucrose and the production of H2S. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks.

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Organisms Ref. No Result
Escherichia coli NCTC 12241 Acid & Gas
Salmonella typhimurium NCTC 12023 Acid & Gas H2S
Pseudomonas aeruginosa NCTC 12903 No Change