KM0003 – Sabouraud Dextrose Agar

//KM0003 – Sabouraud Dextrose Agar

KM0003 – Sabouraud Dextrose Agar

Sabouraud Dextrose Agar is one of several media available for theisolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud Dextrose Agar is a modification of a medium originally described by Sabouraud. The tryptone and meat peptone provide the nitrogen and vitamins required for organism growth in Sabouraud Dextrose Agar. The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties. Higher levels of selectivity can be achieved against bacterial species by the addition of chloramphenicol.
NB: This is a basic medium only and contains no additional supplements.

Related Supplements :
LS0050 Chloramphenicol Selective Supplement

SKU: KM0003 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Saccharomyces cerevisiae NCPF 3178 Growth
Aspergillus brasiliensis NCPF 22785 Growth, white mycelia with black spores

 

Formula*

g/L
Tryptone 5.0
Meat peptone 5.0
Dextrose 40.0
Agar 15.0
Total 65.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 65g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.
NB: Avoid overheating this medium as the acidic pH may result in a softer final medium.