KM0004 – CLED SI Agar

//KM0004 – CLED SI Agar

KM0004 – CLED SI Agar

CLED SI Agar

Cystine Lactose Electrolyte Deficient Single Indicator (CLED SI) Agar is based on Mackey and Sandy’s formulation and is popular for culturing urine specimens in the clinical laboratory. The reduced number of electrolyte level prevents swarming of Proteus spp. The peptone and beef extract is the source of the required nitrogen, carbon and vitamins. Lactose is a carbohydrate. The inclusion of bromothymol blue as a pH indicator allows easy differentiation of lactose and non-lactose fermenting organisms. L-Cystine is also present to benefit those organisms that have a particular cystine requirement.

 

SKU: KM0004 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth, yellow colonies
Escherichia coli NCTC 12241 Growth, yellow colonies
Proteus mirabilis NCTC 10975 Growth, blue Colonies (No Swarming)

 

Formula*

g/L
Peptone 8.0
Beef extract 3.0
Lactose 10.0
L-Cystine 0.128
Bromothymol blue 0.02
Agar 15.0
Total 36.2
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 36.2g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.