KM0005 – CLED DI Agar

//KM0005 – CLED DI Agar

KM0005 – CLED DI Agar

Bevis modified Mackey and Sandy’s original medium by introducing a double indicator to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci spp.
Cystine Lactose Electrolyte Deficient Double Indicator (CLED DI) is popular for urine culture in the clinical laboratory. The reduced number of electrolytes prevents swarming of Proteus spp. The peptone and beef extract is the source of the required nitrogen, carbon and vitamins. Lactose is a carbohydrate. The inclusion of bromothymol blue and Andrade’s as indicators allows easy
differentiation of lactose and non-lactose fermenting organisms. L-Cystine is also present to benefit those organisms that have a particular cystine requirement. Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium.

 

SKU: KM0005 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth, yellow colonies
Escherichia coli NCTC 12241 Growth, red/orange colonies with red halo
Proteus mirabilis NCTC 10975 Growth, blue Colonies (No Swarming)

 

Formula*

g/L
Peptone 8.0
Beef extract 3.0
Lactose 10.0
L-cystine 0.0128
Anrade’s Indicator 0.08
Bromothymol Blue 0.02
Agar 15.0
Total 36.2
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 36.2g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes Heat with frequent agitation and boil for one minute to completely
dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile
containers.