KM0007 – Primary UTI Agar

//KM0007 – Primary UTI Agar

KM0007 – Primary UTI Agar

Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed with the aim of simplifying the differentiation and presumptive identification of the main organisms usually found in Urinary Tract Infections (UTI). The peptone is the source of the required nitrogen, carbon and vitamins. Based on the traditional CLED medium, to prevent the swarming of Proteus spp., two chromogens are present in the medium. One allows the detection of enterococci giving rise to blue colonies whilst the second results in purple colonies of E. coli. Phenylalanine and tryptophan are also included as indicators of tryptophan deaminase activity producing brown colonies of Proteus spp.

Related Supplements :
SHS500 Sterile Horse Serum 500ml

SKU: KM0007 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Recommended Incubation

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth, cream colonies
Escherichia coli NCTC 12241 Growth, purple colonies
Enterobacter aerogenes NCTC 10006 Growth, metallic blue colonies
Proteus mirabilis NCTC 10975 Growth, colourless colonies with brown halo
Enterococcus faecalis NCTC 12697 Growth, blue/turquoise colonies

 

Formula*

g/L
Peptone 20.5
Chormogenic mix 4.0
Agar 12.5
Total 37.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 37g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.