KM0008 – T.C.B.S Medium

//KM0008 – T.C.B.S Medium

KM0008 – T.C.B.S Medium

Thiosulphate citrate bile salts sucrose (TCBS) agar is a selective medium used in microbiology laboratories to isolate Vibrio spp. The formulation was developed by Kobayashi et al. which was modified from Nakanishi’s formulation. The yeast extract and peptone are the source of the required nitrogen, carbon and vitamins. TCBS agar contains high concentrations of sodium thiosulphate, sodium citrate and ox bile to inhibit the growth of Gram-positive organisms and suppress coliforms. Sucrose is included as a fermentable carbohydrate for the metabolism of Vibrio spp. Sodium chloride maintains the osmotic balance in the medium. Sodium thiosulphate also serves as a sulphur source and, in combination with ferric citrate, detects hydrogen
sulphide production. Thymol blue and bromothymol blue are included as indicators of pH changes.

SKU: KM0187 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Vibrio parahaemolyitcus NCTC 10885 Growth, green colonies
Vibrio furnissii NCTC 12218 Growth, yellow colonies
Vibrio chlorerae NCTC 11348 Growth, yellow colonies
Escherichia coli NCTC 12241 Inhibited

 

Formula*

g/L
Yeast extract 5.0
Peptone 10.0
Sodium thiosulphate 10.0
Sodium citrate 10.0
Ox bile 8.0
Sucrose 20.0
Sodium chloride 10.0
Ferric citrate 1.0
Bromothymol blue 0.04
Thymol blue 0.04
Agar 11.0
Total 85.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 85g/l of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation until boiling. Cool to 45-50°C and aseptically dispense into appropriate sterile containers
NB: Protracted boiling or prolonged holding at elevated temperature induces precipitation.