KM0009 – Helicobacter Pylori Agar

//KM0009 – Helicobacter Pylori Agar

KM0009 – Helicobacter Pylori Agar

This is a selective medium for the isolation of Helicobacter pylori from clinical samples. The medium is based on a modification of Campylobacter CCDA Blood Free Medium with charcoal, ferrous sulphate and sodium pyruvate replacing the horse blood. The medium is made selective by the addition of vancomycin and cefsulodin, to suppress other bacteria, and amphoteracin to inhibit yeasts. Horse serum (10%) is also added to promote optimum growth of Helicobacter spp.

Related Supplements :
LS0031 Helicobacter pylori Selective Supplement, SHS500 Sterile Horse Serum 500ml

SKU: KM0009 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Helicobacter pylori NCTC 11916 Growth, grey colonies
Enterococcus faecalis NCTC 12697 Inhibited
Candida albicans NCPF 3179 Inhibited

 

Formula*

g/L
Peptone 23.0
Sodium chloride 5.0
Charcoal 4.0
Ferrous sulphate 0.25
Sodium pyruvate 0.25
Agar 12.0
Sodium carbonate 0.5
Total 45.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 45 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C. and add two vials of Helicobacter pylori selective supplement (LS0031) and 10% horse serum, mix well and aseptically dispense into appropriate sterile containers.