This is a selective medium for the isolation of Helicobacter pylori from clinical samples. The medium is based on a modification of Campylobacter CCDA Blood Free Medium with charcoal, ferrous sulphate and sodium pyruvate replacing the horse blood. The medium is made selective by the addition of vancomycin and cefsulodin, to suppress other bacteria, and amphoteracin to inhibit yeasts. Horse serum (10%) is also added to promote optimum growth of Helicobacter spp.
KM0009 – Helicobacter Pylori Agar
|Shelf Life (days)|
|Storage Temp (°C)|
|Dehydrated Medium Appearance|
|Prepared Medium Appearance|
|Dehydrated Medium Colour|
|Prepared Medium Colour|
|Helicobacter pylori||NCTC 11916||Growth, grey colonies|
|Enterococcus faecalis||NCTC 12697||Inhibited|
|Candida albicans||NCPF 3179||Inhibited|
|*Adjusted/supplemented as required to meet performance requirements|
Suspend 45 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C. and add two vials of Helicobacter pylori selective supplement (LS0031) and 10% horse serum, mix well and aseptically dispense into appropriate sterile containers.