Yeast extract agar is a nutrient rich medium for the cultivation of non-fastidious bacteria, yeasts and moulds. Recommended for the plate count of microorganisms in water and dairy products.
The yeast extract and peptone acts as a source of nitrogen, amino acids, carbon and vitamins.
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| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation |
| Organisms | Ref. No | Result |
| Escherichia coli | NCTC 12241 | Growth, cream colonies |
| Staphylococcus aureus | NCTC 12981 | Growth, white colonies |
| Bacillus subtilis | ATCC 6633 | Growth, cream colonies |
| g/L | |
| Yeast extract | 3.0 |
| Peptone | 5.0 |
| Agar | 15.0 |
| Total | 23g |
| *Adjusted/supplemented as required to meet performance requirements | |
Suspend 23g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and dispense into appropriate sterile containers.