GC LCAT Selective Supplement
E&O Laboratories Ltd LCAT Selective Supplement (LS0001) is an antibiotic supplement used to enhance the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.
GC LCAT Selective Supplement
Additional Information
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance |
Product Description
| Organisms | Ref. No | Result |
| Neisseria gonorrhoeae | NCTC 8375 | Growth |
| Proteus mirabilis | NCTC 10975 | Inhibited |
| Candida albicans | NCPF 3179 | Inhibited |
| Staphylococcus epidermidis | NCTC 13360 | Inhibited |
Formula
Each vial contains raw materials at a concentration of the following per litre:
| Colistin | 75mg |
| Lincomycin | 3mg |
| Amphotericin B | 2mg |
| Trimethoprim | 5mg |
Procedure
To reconstitute the contents of each vial, aseptically add 2ml of Sterile Distilled Water. Close the vial and gently agitate to complete reconstitution. Avoid frothing and add the contents of the vial to an appropriate volume of sterile base medium of choice (GC Agar Base is recommended) prepared according to the manufacturer’s instructions and allowed to cool to 45°-50°C.
Mix gently but thoroughly and pour aseptically into sterile Petri dishes.
Overnight storage at 2°-8°C is recommended to allow equilibration of the supplement within the medium. For extended storage, plates should be contained in sealed plastic sleeves or similar protective packaging.