LS0002 – G.C. Selective Supplement with VCAT

//LS0002 – G.C. Selective Supplement with VCAT

LS0002 – G.C. Selective Supplement with VCAT

Vancomycin. Colistin, Amphotericin, Trimehtoprim (V.C.A.T)
E&O Laboratories Ltd VCAT Selective Supplement (LS0002) is an antibiotic supplement used to enhance the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.

 

SKU: LS0002 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Dehydrated Medium Colour

Solubility Description

Recommended Incubation

Product Description

Organisms Ref. No Result
 Neisseria gonorrhoeae NCTC 8375 Growth
Neisseria gonorrhoeae NCTC 12900 Growth
Proteus mirabilis NCTC 10975 Inhibited
Candida albicans NCPF 3179 Inhibited
Staphylococcus epidermidis NCTC 13360 Inhibited

 

Formula

Each 500ml vial contains:

Colistin 37.5mg/L
Vancomycin 1.5mg/L
Amphotericin B 1mg/L
Trimethoprim 2.5mg/L

Procedure

To reconstitute the contents of each vial, aseptically add 2ml of Sterile Distilled Water. Close the vial and gently agitate to complete reconstitution. Avoid frothing and the resulting solution should be pale straw/yellow.
Add the contents of the vial to 500ml of sterile base medium of choice (GC Agar Base is recommended) prepared according to the manufacturer’s instructions and allowed to cool to 45 – 50°C. Mix gently but thoroughly and pour aseptically into sterile Petri dishes. Overnight storage at 2 – 8°C is recommended to allow equilibration of the supplement within the medium. For extended storage at 2 – 8°C plates should be contained in sealed plastic sleeves or similar protective packaging.