b-Nicotinamide-Adenine Dinucleotide (N.A.D.) Supplement
E&O Laboratories Ltd N.A.D. Supplement (LS0005) is an enrichment supplement for Haemophilus spp. and Neisseria gonorrhoeae. This supplement can be used in conjunction with sensitivity test agars such as: E&O products PP2148 Iso Sensitivity test agar with 5% Horse Blood and 20mgs/L N.A.D. and PP0972 Mueller Hinton agar with 5 % Horse Blood with 20mgs/L N.A.D.
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| pH | |
| Solubility Description | |
| Recommended Incubation | Incubate in aerobic atmosphere supplemented with CO2 35°C ± 2°C for 18-24 hours |
| Organisms | Ref. No | Result |
| Haemophilius influenzae | ATCC 49247 | Growth |
| Neisseria gonorrhoeae | ATCC 49226 | Growth |
| Streptococcus pneumoniae | ATCC 49619 | Growth |
10mg of Nicotinamide adenine dinucleotide (N.A.D)
Suspend the recommended manufacturer’s amount of dehydrated media in distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 45°- 50°C, and aseptically add the contents of one vial of reconstituted Nicotinamide adenine dinucleotide (N.A.D.) (LS0005).
To reconstitute the N.A.D. vial add 2ml of sterile deionised water. Shake the reconstituted vial gently to dissolve and add to the medium.