PP4085 – Middlebrook 7H10 Selective Agar (25ml)

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PP4085 – Middlebrook 7H10 Selective Agar (25ml)

Middlebrooks 7H10 Selective Medium is an Agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H11 Agar in that it has a lower concentration of Malachite Green, which is said by some workers to make it more suitable for primary isolation.

The medium is complex but includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. Further enrichment is provided by the addition of Oleic Acid, Albumen and Dextrose and it is made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B.

As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria.

 

SKU: PP4085 Categories: ,

Additional Information

Container

Pack Size

10 x 90mm Plates

Shelf Life (days)

Storage Temp (°C)

Appearance

Firm Gel

Colour

Beige

PH

Fill Volume

Product Description

Organisms Ref. No Result
Mycobacteruim smegmatis ATCC 14468 Growth
Candida albicans NCPF 3179 Inhibited
Pseudomonas aeruginosa NCTC 12903 Inhibited
Aspergillus brasiliensis NCPF 2275 Inhibited
Staphylococcus aureus NCTC 12981 Inhibited