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This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia (USP) for antibiotic sensitivity testing of pharmaceutical products. As previously stated Antibiotic Medium No. 1 is used in the performance of antibiotic assays. This medium is prepared according to the specifications detailed in the USP. The use of this medium assures well-defined inhibition zones of the test organisms. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source. -
This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No.32 is a modification of Antibiotic assay medium No.1. (E & O BM4710). This medium is used to develop inoculum of Bacillus subtilis for antibiotic assay used in the test for assaying by the plate assay method. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source. -
This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No. 8 is recommended for preparing inoculum of Bacillus subtilis to be used as a test for assaying Vancomycin by plate assay method. -
This is a variation on sterile isotonic Saline which is suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. The addition of glass beads will allow dense material to be broken down and aid the isolation of any bacteria that may be present in “clumps”. -
Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc. -
This is a very nutritious general-purpose medium suitable for the isolation of most organisms including many fastidious anaerobes. It is particularly recommended for streptococci and neisseria. -
A very nutritious isotonic general purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture. -
This medium is used to detect and/or confirm the presence of coli-aerogenes group of organisms in water, food and dairy laboratories. Bile and Brilliant Green are included in the medium to inhibit gram positive organisms while the coli-aerogenes organisms are identified by the formation of gas during the fermentation of Lactose. The medium can also be used for the confirmation of Escherichia coli by incubating at 44°C. -
Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms. -
A pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective medium. This non-selective, nutritious medium is free from inhibitors and is well buffered to maintain the pH at 7.0 for the incubation period according to ISO 6579 (2002). -
Buffered Sodium Chloride Peptone Diluent is used for the microbial examination of pharmaceutical products, e.g. as a diluent for sample preparation or as a rinsing solution. This formulation complies with the Harmonized USP/EP/JP. -
Columbia Agar is a nutritious general-purpose basal medium capable of supporting the growth of most micro-organisms, including many fastidious organisms that do not require blood. However when further enriched with Sterile Blood, which can be “chocolated” if required, the medium is generally used for the isolation of most clinically significant pathogens. The medium can be made selective for various groups of organisms by the addition of a range of antimicrobial supplements. This formulation complies with the Harmonized USP/EP/JP. -
Prepared from Minced Meat granules overlaid with Fastidious Anaerobe Broth this medium is suitable for the recovery, isolation and storage of the most fastidious anaerobes and is possibly one of the best variations of Cooked Meat Medium available. Fastidious Anaerobe Broth is designed for optimum growth of all anaerobes, with the growth factors Vitamin K, Haemin and L-Cysteine included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate is also present to reduce the ph of the medium and a small amount of Agar to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. If immediately before use a narrow band of reddish/purple is apparent at the surface of the broth this does not indicate that the medium is unsuitable for use. This is due to the action of oxygen on the redox indicator and the medium should be placed in a boiling water bath, with the cap loosened, for about 15 minutes to remove dissolved oxygen. Immediately on removal from the water bath the cap should be tightened and the medium allowed to cool without agitation. -
Prepared from Minced Meat granules overlaid with Brain Heart Infusion Broth this medium is suitable for recovery, isolation and storage of the most fastidious anaerobes as well as many aerobes. -
All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present.
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A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. The addition of glass beads will allow for the maceration of the sample and aid the homogenisation of the suspension. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. This formulation complies with the United States Pharmacopoeia. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. Fluid D has been formulated with the addition of polysorbate 80 to enable the rinsing of products containing lecithin or oil. This formulation complies with the Harmonised USP/EP/JP.
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This medium is intended for use in sterility testing of substances in accordance with the United States Pharmacopoeia and European Pharmacopoeia. It is a general purpose medium for the cultivation of both fastidious anaerobic and aerobic micro-organisms. Sodium Thioglycollate and L-cystine are included to reduce the medium to ensure anaerobiosis and to inactivate mercury and other heavy metallic compounds. A small amount of agar is added to further reduce the update of oxygen. However, in the event of oxidation, the medium will turn pink due to the presence of the resazurin. Providing only 30% of the medium has turned pink, the Eh may be restored by heating the medium (once only) in a boiling water bath. -
For in vitro diagnostic use only. SW0005-M055-3 Strep B Swab Kit contains Lim Broth, a nutritious medium for the enrichment and transport of Group B streptococci along with a sterile swab suitable for collection of patient samples. The product may be used to preserve, transport, and enrich Group B streptococci that may be present in samples obtained from patients by use of the swab. Each pack also contains a sterile swab in an individual peel-pouch. The swab has a polystyrene shaft with a small viscose tip. The shaft has a breakpoint 78mm from the tip allowing the shortened swab to engage in the shaft capture device in the lid and ensure that the swab is removed from the tube when the cap is unscrewed. Lim broth is a nutritious, selective broth utilising the base formulation developed by Todd and Hewitt (1) for the enrichment of Group B streptococci. It is intended as a selective, enrichment broth for Streptococcus agalactiae that may be present is specimens. The device is primarily intended for use in screening pregnant women for the presence of S. agalactiae, which poses a significant risk of infection in new-born infants that may result in serious illness or death. -
A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.
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This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis. -
Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim. It is sometimes used in conjunction with Mueller-Hinton Agar. -
A general purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements. This particular formulation has additional 0.2% Agar added to provide for a firmer medium without loss of efficacy. Generally used to maintain cultures or to check the purity of subcultures from isolation media. -
A general-purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements. -
Based on Nutrient Broth with an additional 2.5% Sodium Chloride this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus especially MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media as described in PHE SMI B29 issue No.6.
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Based on Nutrient Broth with an additional 6.0% Sodium Chloride (Total Sodium Chloride content = 6.5%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media. -
Based on Nutrient Broth with an additional 6.5% Sodium Chloride (Total Sodium Chloride content = 7%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media. -
A general-purpose medium that can be used as a base for carbohydrate fermentation media. It has a high level of Tryptone and is therefore also suitable for use in Indole testing. -
A general-purpose diluent for the preparation of test samples according to ISO/DIS 6887-5. -
A standard biochemical reagent suitable for a variety of uses, primarily the preparation of serial dilutions. -
Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B). -
Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution with added Tween is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B). -
Originally intended for use in surface counting and pour plating techniques this medium can be used as a general purpose medium for the cultivation of most organisms particularly those that are less fastidious in their nutritional requirements. Can also be used as a maintenance medium for stock cultures. -
An osmotically controlled solution for the preparation of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
An osmotically controlled solution for the preparation of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
This is a variation on the common osmotically controlled Ringers solution, where glass beads have been added to enhance macerations in the preparation of suspensions of samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
A long established selective medium for the isolation of yeasts and fungi suitable for use in all areas of Mycology. The low pH (5.6) of the medium inhibits most bacteria and spore structures and pigmentation of fungi are generally well developed on this medium. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the Harmonized USP/EP/JP. -
Sterile Saline (0.45%) is suitable for use in preparation of food samples and/or as a rinse fluid during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory.
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Sterile Isotonic Saline suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. -
Universal Isotonic Saline Solution. -
This product may be used as an alternative pre-treatment solution to lessen the background flora of samples before the testing for Mycobacteria species. This reagent should only be used for samples that routinely produce contaminated cultures after processing with an alkaline digestant. -
A nutritious broth medium formulated by Todd and Hewitt for the production of antigenic streptococcal haemolysin. The broth is also used to cultivate streptococci prior to serological grouping. Normally the use of fermentable sugars in the broth would inactivate the haemolysin due to acid production. This is prevented by the use of buffers to maintain the pH in this broth. -
This is a general-purpose medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products as well as being suitable in all areas of bacteriological investigation. -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). The medium can be incubated under aerobic or anaerobic conditions after use for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. -
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. This is a general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and Soy peptone are the nitrogen sources in the medium. Glucose is the carbon energy source that facilitates organism growth. Sodium chloride maintains osmotic balance; Di-potassium phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth formulation conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
