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Baird Parker Agar with 5% Egg Yolk Tellurite Baird Parker Agar is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. The medium is made highly selective by the inclusion of Lithium Chloride and Potassium tellurite. The Potassium tellurite inhibits most coliforms and is reduced by staphylococci giving rise to black colonies. Glycine and Sodium Pyruvate are included as growth enhancers while the pyruvate also acts as a neutraliser of toxic peroxides. NB: Any black colonies (with or without the halo) on this medium must be confirmed as Staphylococcus aureus by further tests (e.g. Coagulase Test or Latex Agglutination etc.) -
Brilliant Green Agar This medium is intended for use in the isolation of Salmonellae other than Salmonella typhi. Although it can be used as a primary isolation medium it is not recommended for this purpose and is generally used for subculture from selective enrichment media. It should be noted that the medium is highly selective and therefore not suited to the isolation of Salmonella from samples where the numbers may be low. NB: It is not recommended that this medium be used for the isolation of Salmonella typhi and Shigella spp. -
A highly nutritious medium used for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophillus species from a variety of clinical specimens. The media is further enriched with Suplex (Polyvitex) that provides vitamins, amino acids, co-enzymes, glucose and other factors which improve the growth of Neisseria and Haemophillus species. -
A highly nutritious medium enriched with Horse Blood, where the blood has been “chocolated” by heating the medium to 60°C. Suitable for the isolation of most pathogens including the most fastidious and is particularly useful for the cultivation of Haemophilus spp. and Neisseria spp. -
Chocolate Agar with 7% Horse Blood & Bacitacin A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 70°C. Suitable for the isolation of most pathogens including many fastidious organisms the addition of Bacitracin makes it is particularly suitable for the selective isolation of Haemophilus spp. -
CLED Agar Mackey and Sandy’s formulation this medium is popular for Urine Culture in the clinical laboratory. The lack of electrolytes inhibits the spreading of Proteus spp. and Bromothymol Blue indicator allows easy differentiation of Lactose and Non-Lactose fermenting organisms. Cystine is also present to benefit those organisms that have a particular Cystine requirement. Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium. -
CLED Agar (Bevis) A modification by Bevis of the original CLED medium of Mackey & Sandys. This formulation uses a double indicator system (Andrade’s (Acid fuchsin) and Bromothymol blue) to improve differentiation of Lactose and Non-lactose fermenting organisms. The lack of Sodium Chloride also prevents the swarming of Proteus spp. -
Colorex™Acinetobacter MDR is a chromogenic medium for the detection of multi-drug resistant (MDR) Acinetobacter spp. Positive colonies exhibit a distinct red colouration with a pale grey centre enabling easy interpretation amongst blue, violet or colourless colonies that may be produced by other Gram –ve bacteria. Gram +ve bacteria and yeast are inhibited on this medium. Limitations: 1. Some Pseudomonas, Stenotrophomonas and Burkholderia spp. may form pale red colonies on this medium but are readily distinguishable due to differences in colonial morphology compared to the Acinetobacter spp. An oxidase test will readily differentiate any Pseudomonas spp. 2. Some Enterobacteriaceae isolates may form blue colonies on this medium. 3. Definitive MDR characterisation may require additional antibiotic susceptibility testing. -
A basic general-purpose blood free medium, capable of supporting the growth of most micro-organisms, including many fastidious organisms that do not require blood. -
Fastidious Anaerobe Agar with 7% Horse Blood Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes including fastidious organisms. The formulation is complex and includes detoxification agents (Starch & Sodium Bicarbonate) growth enhancing agents (Cysteine, Arginine, Vitamin K, Sodium Succinate, Glucose and pyrophosphate) as well as Haemin to encourage pigment production in Porphyromonas melaninogenicus. Sodium Pyruvate is also included to help neutralise Hydrogen Peroxide. In this instance the medium is further enriched by the addition of 7% Horse Blood. -
Fastidious Anaerobe Agar (FAA) with 7% Horse Blood & Neomycin (75mg/L) Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes including fastidious organisms. The formulation is complex and includes detoxification agents (Starch & Sodium Bicarbonate) as well as growth enhancing agents (Cysteine, Arginine, Vitamin K, Sodium Succinate, Glucose and pyrophosphate) as well as Haemin to encourage pigment production in Porphyromonas melaninogenicus. Sodium Pyruvate is also included to help neutralise Hydrogen Peroxide. In this instance the medium is made more selective by the inclusion of 75mg/L of Neomycin to inhibit most enteric organisms and further enriched by the addition of 7% Horse Blood. -
This is a selective medium for the isolation and enumeration of enterococci (Group D streptococci) primarily in food although it has found uses in other areas of bacteriology. The medium is made selective by the inclusion of Kanamycin and Sodium Azide while Aesculin and Ferric Ammonium Citrate act as the indicator system. -
One of several media available as a selective identification medium for the isolation and identification of Listeria monocytogenes in food & clinical laboratories. Using Columbia Agar as the base Lithium Chloride is included to inhibit enterococci and Acriflavine to inhibit some other gram positive and gram negative organisms that may be present in such specimens. It is made further selective by the addition of the antimicrobials Cefoxitin, Colistin & Fosfomycin with Amphotericin included to inhibit any yeasts present. Aesculin is present as an indicator since Listeria monocytogenes will hydrolyse it and the associated reaction with the Ferric Ammonium Citrate gives rise to a black precipitate around the colonies. -
A basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms particularly those that do not require the addition of blood or other enrichment. -
This is an established medium, with a neutral pH, used for the enumeration of Yeasts and Moulds -
Plate Count Agar (APHA) (Standard Methods Agar, Tryptone Glucose Yeast Agar) This medium is formulated to A.P.H.A. specification and intended for use in food, dairy and water bacteriology to perform Total Viable Counts. The agar is of high gel strength and is therefore suitable for use in pour plate techniques as well as surface inoculation. -
Triphenyltetrazolium Chloride Soya Tryptone (TSAT) Agar Complete Triphenyltetrazolium Chloride (TTC) has been added as an indicator to various media, and recommended by several workers as being helpful in the early recognition and identification of a variety of bacteria including Escherichia coli, Vibrio parahaemolyticus and enterococci. This particular formulation is based on a Tryptone Soya Agar with added Sucrose and is particularly useful when performing counts on food and food product samples. Many of the enterobacteriaceae and enterococci will reduce the TTC to a formazan which colours the colonies deep red making them easier to distinguish and identify. The presence of the Sucrose can also assist in the differentiation of Sucrose fermenting and non-fermenting strains. -
Violet Red Bile Glucose Agar Violet Red Bile Glucose Agar is a selective medium for the isolation and enumeration of enterobacteriacae in food products. It is a modification of the original Violet Red Bile Agar (PP1150) with the Lactose being replaced with Glucose. As all members of the enterobacteriacae ferment Glucose it allows for a wider range of organisms to be detected. The medium is made selective by the inclusion of Bile Salts and Crystal Violet to inhibit gram-positive and other non-enteric organisms.
