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  • This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. Designed for use with NaOH treated samples which have sterile distilled water added at the end of the designated treatment time to "dilute" the effect of NaOH. NB: this media will NOT isolate M.bovis which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
  • This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp. Designed for use with NaOH treated samples which have sterile distilled water added at the end of the designated treatment time to "dilute" the effect of NaOH. This media will isolate most common mycobacteria including M.bovis. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
  • This is a 25ml fill selective medium for the isolation of Actinomyces spp from clinical specimens. Based on Fastidious Anaerobe Agar enriched with 7% Horse Blood, the medium has been made selective by the inclusion of Nalidixic Acid to inhibit most aerobes, particularly gram-negative bacilli, and Metronidazole to suppress other anaerobes.
  • E&O Laboratories Ltd Actinomycete Selective Supplement (LS0015) is an antibiotic supplement used to enhance the isolation of Actinomyces spp.  
  • Alkaline peptone water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. This medium may also be used for the enrichment of Vibrio spp. from food and water samples. First developed by Shread, Donovan and Lee as an enrichment broth for the growth of Aeromonas spp., Cruickshank showed that with a higher pH the medium can be used for the enrichment of Vibrio spp. The peptone is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance. The high pH of the medium inhibits most enteric organisms for at least 24 hours.
  • Alkaline Peptone Water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. The high pH of the medium inhibits most enteric organisms for at least 24 hours. The medium is heavily inoculated with faeces and after not more than 8 hours incubation a loopful from the top of the medium is sub cultured onto TCBS Agar. This enrichment medium is also used for food and water testing.
  • Alpha Syrup Bottles with 28mm cap Assorted capacities available with these associated container codes: 1L - 1 litre 500 - 500ml 300 - 300ml 125 - 125ml 060 - 60ml
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  • Ampicillin Selective Supplement (100mgs/L) E&O Laboratories Ltd Ampicillin Selective Supplement can be used with LB Agar for selective cultivation of E. coli strains that contain plasmids conferring ampicillin resistance. Ampicillin is an antibiotic used to treat a number of bacterial infections. It is a beta-lactam antibiotic that is part of the amino penicillin family. It is active against many Gram-positive and Gram-negative bacteria.  
  • This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia (USP) for antibiotic sensitivity testing of pharmaceutical products. As previously stated Antibiotic Medium No. 1 is used in the performance of antibiotic assays. This medium is prepared according to the specifications detailed in the USP. The use of this medium assures well-defined inhibition zones of the test organisms. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source.
  • This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No.32 is a modification of Antibiotic assay medium No.1. (E & O BM4710). This medium is used to develop inoculum of Bacillus subtilis for antibiotic assay used in the test for assaying by the plate assay method.  Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source.
  • This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium  No. 8 is recommended for preparing inoculum of Bacillus subtilis to be used as a test  for assaying Vancomycin by plate assay method.
  • B.cepacia Selective Supplement E&O Laboratories Ltd Burkholderia Selective Supplement (LS0125) is an antibiotic supplement used to enhance the isolation of Burkholderia cepacia (formerly known as Pseudomonas cepacia).  
  • Bacillius cereus Selective Agar (PEMBA) This is a medium for the selective isolation and enumeration of Bacillus cereus in food samples. It is said to be particularly suitable for the detection of small numbers of Bacillus cereus particularly in the presence of other contaminating organisms. The medium is made selective by the inclusion of Polymixin and Sodium Pyruvate is also present which is said to improve Egg Yolk precipitation and enhance sporulation. As Bacillus cereus is Mannitol Negative the colonies are bluish in colour, due to the presence of the Bromothymol Blue Indicator, with a surrounding precipitate of the same colour due to Lecithinase production (from the Egg Yolk). NB:  It should be noted that some Proteus spp. and gram positive cocci may grow on this medium.
  • Bacillus cereus (MYP) agar is intended for the selective enumeration of Bacillus cereus in food samples. This medium utilizes two reactions namely mannitol fermentation and lecithinase production to differentiate Bacillus cereus from other related species. As B. cereus is mannitol negative the colonies are pink in colour due to the presence of the phenol red pH indicator. Lecithinase production (from the addition of egg yolk) is indicated by a white precipitate around the colonies. This medium meets the requirements of ISO 7932:2004. Beef extract and peptone provide the required carbon, nitrogen and vitamins. Sodium chloride helps maintain the osmotic balance and phenol red is the pH indicator. Mannitol is a fermentable carbohydrate. The medium is made selective by the inclusion of polymyxin B sulphate (LS0020). NB: This is a basic medium only and contains no additional supplement. It should be noted that some Proteus spp. and Gram-positive cocci may grow on this medium. Related Supplements : LS0020 Bacillus cereus Selective Supplement, BM0140 Egg Yolk Emulsion
  • Bacillus cereus agar (PEMBA) is used for the selective isolation and enumeration of Bacillus cereus in food samples. It is said to be particularly suitable for the detection of small numbers of Bacillus cereus particularly in the presence of other contaminating organisms. Bacillus cereus agar (PEMBA) is based on the formulation developed by Holbrook and Anderson. The peptone provides the required carbon, nitrogen and vitamins. Mannitol is a fermentable carbohydrate. Bromothymol blue is a pH indicator used to detect mannitol fermentation. Sodium chloride maintains the osmotic balance. Magnesium sulphate provides divalent cations and sulphate. Di-sodium hydrogen phosphate and potassium dihydrogen phosphate are buffering agents. Egg yolk emulsion (BM0140) must be added to this media to assess lecithinase production. Sodium pyruvate is present to improve egg yolk precipitation and enhance B. cereus sporulation. The medium is made selective by the inclusion of polymyxin B sulphate (LS1051).
  • Bacillus cereus Selective Supplement E&O Laboratories Ltd Bacillus Cereus Selective Supplement (LS0020) is an antibiotic supplement used to enhance the selective isolation of Bacillus cereus from foodstuffs and clinical specimens.
  • Bacitracin Selective Supplement E&O Laboratories Ltd Bacitracin Supplement (LS0012) is an antibiotic supplement used to enhance the isolation of Haemophilus species.
  • Bacteriological peptone is an economical source of nutrients provided by a balanced mixture of meat peptones and tryptone.The growth requirements of most non fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.  
  • Baird Parker agar is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. Enzymatic digest of casein, meat extract and yeast extract provide the required carbon, nitrogen and vitamins. The medium is made selective by the inclusion of lithium chloride and the addition of potassium tellurite. Glycine and sodium pyruvate are included as growth enhancers while the pyruvate also acts as a neutraliser of toxic peroxides. Coagulase positive staphylococci are differentiated by the addition of egg yolk tellurite (BM0530) due to their ability to break down the egg yolk. Clear zones around colonies form due to the proteolytic action of lecithinase. A secondary opaque zone, surrounding presumptive positive colonies, may also form due to lipase activity. Potassium tellurite is reduced by staphylococci giving rise to blackening of colonies. NB: Any black colonies (with halo (typical) or without the halo (atypical)) on this medium must be confirmed as Staphylococcus aureus by further tests (e.g. coagulase test or latex agglutination etc.) Related Supplements : BM0530 Egg Yolk Tellurite
  • Baird Parker Agar with 5% Egg Yolk Tellurite Baird Parker Agar is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. The medium is made highly selective by the inclusion of Lithium Chloride and Potassium tellurite. The Potassium tellurite inhibits most coliforms and is reduced by staphylococci giving rise to black colonies. Glycine and Sodium Pyruvate are included as growth enhancers while the pyruvate also acts as a neutraliser of toxic peroxides. NB: Any black colonies (with or without the halo) on this medium must be confirmed as Staphylococcus aureus by further tests (e.g. Coagulase Test or Latex Agglutination etc.)
  • This is a variation on sterile isotonic Saline which is suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. The addition of glass beads will allow dense material to be broken down and aid the isolation of any bacteria that may be present in “clumps”.
  • KM8006

    Beef Extract

    Beef extract is sourced from bovine tissue and supports the nutritive needs of microorganisms. Providing a mixture of amino acids, peptides, minerals and vitamins this product is recommended for use in microbiological culture media for the examination of water, milk and other materials.  
  • Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.
  • Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.
  • Bile Aesculin Agar Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.
  • Blood agar base No. 2 is a general-purpose medium enriched with various concentrations of horse or sheep blood and is suitable for the isolation of most organisms including many fastidious anaerobes of clinical significance. Haemolysis observations may vary with the type of blood being used. Previous studies have shown that sheep blood provides the most reliable colony and haemolysis characteristics. The peptone, yeast extract and liver digest act as nitrogen, carbon and vitamin sources in this medium. Sodium chloride maintains osmotic balance. Related Supplements : Defibrinated Sheep Blood, Defibrinated Horse Blood, LS0008 Staph/Strep Selective Supplement, LS0017 Neomycin Selective Supplement
  • A general-purpose medium enriched with 7% Horse Blood, suitable for the isolation of most organisms including many fastidious anaerobes of clinical significance.
  • 2 port designed blood bags for large volume capacity Volume(s) : 1L, 4L, 10L
  • A general purpose medium enriched with 5% Defibrinated Sheep Blood, suitable for the isolation of most organisms including many fastidious anaerobes.
  • Bordetella Selective Supplement Bordetella Selective Supplement LS0018 is an antibiotic supplement used to enhance the culture of Bordetella pertussis.  
  • Brain heart infusion agar is very nutritious general-purpose medium and is suitable for the isolation of most micro-organisms including many fastidious organisms. The formulation is a modification of that from Rosenow and Hayden. The medium is not recommended for the determination of haemolytic reactions because of the glucose content. The nitrogen, vitamin and carbon sources are supplied by the Brain-Heart infusion solids and peptone. Glucose serves as the carbohydrate source and sodium chloride aids in maintaining the osmotic balance. A phosphate buffer, disodium hydrogen phosphate, is incorporated to help neutralize any acids produced as a result of glucose utilization and thus maintain viability of the organisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result may ‘auto-sterilize’ the culture.
  • This is a very nutritious general-purpose medium suitable for the isolation of most organisms including many fastidious anaerobes. It is particularly recommended for streptococci and neisseria.
  • A very nutritious isotonic general purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture.
  • Brain heart infusion broth is very nutritious isotonic medium with a low concentration of glucose to stimulate early growth. The formulation is a modification of that from Rosenow and Hayden. Brain heart infusion broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with the appropriate enrichment, is suitable as a base for blood culture medium. The nitrogen, vitamin and carbon sources are supplied by the Brain-Heart infusion solids and peptone. Glucose serves as the carbohydrate source and sodium chloride aids in maintaining the osmotic balance. A phosphate buffer, disodium hydrogen phosphate, is incorporated to help neutralize any acids produced as a result of glucose utilization and thus maintain viability of the organisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result may ‘auto-sterilize’ the culture.  
  • An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms.
  • A very nutritious isotonic general-purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. This particular formulation also has added Glycerol to act as cryopreservative if the medium is used for the long term frozen storage of microorganisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture.
  • An enriched general purpose broth being an isotonic medium with Tryptose providing a wide range of substrates. The medium is further enriched with 10% horse serum for more fastidious organisms and as an enrichment broth.
  • Brain-heart infusion solids is a dehydrated infusion of porcine brains and heart. This product can be used in preparing microbiological culture media providing nitrogen, vitamins, minerals and amino acids.  
  • Brazier's CCEY Agar with 1% Lysed Horse Blood - Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light.
  • Brilliant green agar is for the selective isolation of Salmonella spp., other than S. typhi. Brilliant green agar was first cited by Kristensen et al. in 1925 but was subsequently modified by the Netherlands Institute for Public Health. Brilliant green agar is generally used in conjunction with XLD agar (KM0013) as a secondary plating medium for subculture from selective enrichment media in food and environmental testing. It should be noted that the medium is highly selective and therefore not suited to the isolation of Salmonella spp. from samples where the numbers may be low. Beef extract, peptone and yeast extract provide the required carbon, nitrogen and vitamins. Di-sodium hydrogen phosphate and sodium di-hydrogen phosphate are buffering agents. Lactose and sucrose are fermentable carbohydrates. Phenol red is a pH indicator and turns the medium yellow during lactose and/or sucrose fermentation. Brilliant green inhibits Gram-positive bacteria and Gram-negative bacilli other than Salmonella spp. NB: It is not recommended that this medium be used for the isolation of Salmonella typhi or Shigella spp. Related Supplements : LS0024 Novobiocin Supplement (20mgs/L)
  • This medium is used to detect and/or confirm the presence of coli-aerogenes group of organisms in water, food and dairy laboratories. Bile and Brilliant Green are included in the medium to inhibit gram positive organisms while the coli-aerogenes organisms are identified by the formation of gas during the fermentation of Lactose. The medium can also be used for the confirmation of Escherichia coli by incubating at 44°C.
  • Brilliant Green Agar This medium is intended for use in the isolation of Salmonellae other than Salmonella typhi. Although it can be used as a primary isolation medium it is not recommended for this purpose and is generally used for subculture from selective enrichment media. It should be noted that the medium is highly selective and therefore not suited to the isolation of Salmonella from samples where the numbers may be low. NB:  It is not recommended that this medium be used for the isolation of Salmonella typhi and Shigella spp.
  • Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms.
  • This is a modification of the original Nitrate Reduction Broth which is generally used as one of a series of identification tests for the enterobacteriaceae group of organisms. In addition to allowing the testing of Nitrate Reduction this formulation also contains Agar making it possible to concurrently determine motility. The medium is recommended for use in the confirmatory testing of Clostridium perfringens in water samples. The medium is inoculated by “stabbing” the test organism into the medium, using an inoculating needle or straight wire, and after appropriate incubation motility is demonstrated by diffusion of the organism from the line of inoculation into the medium. The Nitrate Reduction Test is a test for the presence of the enzyme nitrate reductase which, in the presence of an appropriate electron donor, reduces nitrate to nitrite. Following incubation “Nitrate Reagent” is added to the medium and a positive reaction is indicated by the formation of a red colour. For full details of the test method reference should be made to appropriate publications.
  • A pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective medium. This non-selective, nutritious medium is free from inhibitors and is well buffered to maintain the pH at 7.0 for the incubation period according to ISO 6579 (2002).