Antibiotic Supplements

Antibiotic Supplements products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

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  • Clostridium perfringens Selective Supplement E&O Laboratories Ltd Clostridium Perfringens (TSC) Selective Supplement (LS0023) is an antibiotic supplement used to enhance the selective isolation of Clostridium perfringens from clinical specimens and foodstuffs.  
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  • Ferric Ammonium Citrate (FAC) Supplement E&O Laboratories Ltd Ferric Ammonium Citrate (LS5004) is a supplement used in the isolation of Listeria spp. with Fraser Broth. Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. The medium is intended for the isolation of Listeria spp. from food and environmental samples when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. It is generally accepted that the USDA-FSIS two stage enrichment method employing UVM primary and secondary enrichment broths is the most suitable for the examination of meat products.
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  • Half Fraser Broth Selective Supplement E&O Laboratories Ltd Half Fraser Selective Supplement (LS5003) is selective supplement for the primary enrichment of Listeria spp from food and environmental samples when used with Fraser Broth.Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. It contains ferric ammonium citrate and lithium chloride. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. Fraser Broth has proven to be remarkably accurate in detecting Listeria spp. in food and environmental samples. All Listeria spp. hydrolyse aesculin to aesculetin. Aesculetin reacts with ferric ions which results in blackening. Another possible advantage to the addition of ferric ammonium citrate is that it has been shown that ferric ions enhance the growth of Listeria monocytogenes. Lithium chloride is included in the medium to inhibit the growth of enterococci which can also hydrolyse aesculin. Half Fraser Broth is a modification of Fraser Broth which contains half of the concentration of nalidixic acid and acriflavine hydrochloride to aid in the recovery of stressed cells. Half Fraser Broth is used as the primary enrichment broth in the ISO methodology for the detection of Listeria.
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  • Fraser Broth Selective Supplement E&O Laboratories Ltd Fraser Broth Selective Supplement (LS5002) is an antibiotic supplement used to enhance the selective isolation of Listeria spp. in combination with Fraser Broth.  
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  • Modified Campylobacter (Bolton) Selective Supplement E&O Laboratories Ltd Campylobacter Modified (Bolton) Selective Supplement (LS1054) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens.  
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  • Primary Listeria Selective Supplement E&O Laboratories Ltd Primary Listeria Selective Supplement (LS0210) is a selective mixture used to supplement Primary Listeria Selective Agar (E&O PP7025) in order to facilitate the isolation of Listeria monocytogenes in foodstuffs and other samples.  
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  • Cefotaxime Selective Supplement E&O Laboratories Ltd Cefotaxime Selective Supplement can be used with MacConkey Agar for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing organisms with the aim of simplifying the differentiation and presumptive identification of Lactose & Non-Lactose fermenting organisms. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. Sodium Chloride is omitted from this medium to provide an electrolyte deficient medium that prevents Proteus spp. from spreading.
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  • B.cepacia Selective Supplement E&O Laboratories Ltd Burkholderia Selective Supplement (LS0125) is an antibiotic supplement used to enhance the isolation of Burkholderia cepacia (formerly known as Pseudomonas cepacia).  
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  • Chloramphenicol Selective Supplement (100mgs/L) E&O Laboratories Limited Chloramphenicol Selective Supplement (LS0050) is an antibiotic supplement used for the selective isolation of yeasts and moulds from food, environmental and clinical specimens.  
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  • PALCAM Selective Supplement E&O Laboratories Ltd PALCAM Selective Supplement (LS0038) is a selective mixture used to supplement PALCAM agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from food samples.  
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  • Kanamycin Selective Supplement (50mgs/L) E&O Laboratories Ltd Kanamycin Selective Supplement can be used with LB Agar for selective cultivation of E. coli and other organisms that contain plasmids conferring kanamycin resistance. Kanamycin is an aminoglycoside bacteriocidal antibiotic used to treat a wide variety of infections Kanamycin is isolated from the soil bacterium Streptomyces kanamyceticus.  
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  • Ampicillin Selective Supplement (100mgs/L) E&O Laboratories Ltd Ampicillin Selective Supplement can be used with LB Agar for selective cultivation of E. coli strains that contain plasmids conferring ampicillin resistance. Ampicillin is an antibiotic used to treat a number of bacterial infections. It is a beta-lactam antibiotic that is part of the amino penicillin family. It is active against many Gram-positive and Gram-negative bacteria.  
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  • Helicobacter pylori Selective Supplement E&O Laboratories Ltd Helicobacter pylori Selective Supplement can be used with Helicobacter pylori Base Agar (E&O product KM0009).This selective supplement is composed of 2 antibiotics and one antifungal agent. These agents inhibit the background flora and allow the cultivation of Helicobacter pylori. Helicobacter pylori, previously named Campylobacter pylori, is a Gram-negative, microaerophilic bacterium found in the stomach, and may be present in other parts of the body, such as the eye.
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  • Listeria Oxford Selective Supplement E&O Laboratories Ltd Listeria Oxford Selective Supplement (LS0030) is a selective mixture used to supplement Listeria Oxford agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from clinical and food samples.  
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  • Pseudomonas CFC Selective Supplement E&O Laboratories Ltd Pseudomonas CFC Selective Supplement (LS0026) is an antibiotic supplement used to enhance the selective isolation of pseudomonads primarily from food and environmental samples.  
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  • Novobiocin Supplement (20mgs/L) E&O Laboratories Ltd Novobiocin Selective Supplement can be used to enhance the isolation of Salmonella spp. by motility enrichment and the selective enrichment of E.coli Serogroup O157 in food and faeces samples and also in the isolation of Salmonella typhimurium at 40mgs/L when used with Muller-Kauffmann Tetrathionate (MKTTn) Broth.  
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