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Vancomycin. Colistin, Amphotericin, Trimehtoprim (V.C.A.T) E&O Laboratories Ltd VCAT Selective Supplement (LS0002) is an antibiotic supplement used to enhance the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis. -
GC LCAT Selective Supplement E&O Laboratories Ltd LCAT Selective Supplement (LS0001) is an antibiotic supplement used to enhance the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.
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Half Fraser Broth Selective Supplement E&O Laboratories Ltd Half Fraser Selective Supplement (LS5003) is selective supplement for the primary enrichment of Listeria spp from food and environmental samples when used with Fraser Broth.Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. It contains ferric ammonium citrate and lithium chloride. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. Fraser Broth has proven to be remarkably accurate in detecting Listeria spp. in food and environmental samples. All Listeria spp. hydrolyse aesculin to aesculetin. Aesculetin reacts with ferric ions which results in blackening. Another possible advantage to the addition of ferric ammonium citrate is that it has been shown that ferric ions enhance the growth of Listeria monocytogenes. Lithium chloride is included in the medium to inhibit the growth of enterococci which can also hydrolyse aesculin. Half Fraser Broth is a modification of Fraser Broth which contains half of the concentration of nalidixic acid and acriflavine hydrochloride to aid in the recovery of stressed cells. Half Fraser Broth is used as the primary enrichment broth in the ISO methodology for the detection of Listeria.
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Helicobacter pylori Selective Supplement E&O Laboratories Ltd Helicobacter pylori Selective Supplement can be used with Helicobacter pylori Base Agar (E&O product KM0009).This selective supplement is composed of 2 antibiotics and one antifungal agent. These agents inhibit the background flora and allow the cultivation of Helicobacter pylori. Helicobacter pylori, previously named Campylobacter pylori, is a Gram-negative, microaerophilic bacterium found in the stomach, and may be present in other parts of the body, such as the eye.
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Kanamycin Selective Supplement (50mgs/L) E&O Laboratories Ltd Kanamycin Selective Supplement can be used with LB Agar for selective cultivation of E. coli and other organisms that contain plasmids conferring kanamycin resistance. Kanamycin is an aminoglycoside bacteriocidal antibiotic used to treat a wide variety of infections Kanamycin is isolated from the soil bacterium Streptomyces kanamyceticus.
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E&O Laboratories Ltd Legionella Growth Supplement (LS0053) is an enrichment supplement used to enhance the isolation of Legionella spp. in combination with Charcoal Yeast Extract (CYE) agar. CYE Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of containing the bacteria.
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E&O Laboratories Ltd Legionella BMPA Supplement (LS0156) is used in the identification of suspected colonies of Legionella spp. when used to prepare Legionella BMPA agar. Legionella BMPA Supplement contains cefamandole and polymyxin B to inhibit most Gram-positive and Gram-negative organisms.
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E&O Laboratories Ltd Legionella GVPC Selective Supplement (LS0052) is used in the culture of suspected colonies of Legionella spp. Colonies of Legionella should be visible after 2 to 5 days incubation on BCYE agar with growth supplement and appear light blue to blue-grey in colour. Colonies suspected of being Legionella should be Gram-stained and subcultured to a fresh Legionella agar plate and to a Legionella agar plate not containing L-Cysteine. Gram-negative organisms that grow on Legionella Agar but fail to grow on Legionella agar (without L-Cysteine) may be presumptively identified as Legionella species. Definitive identification is performed on the basis of growth, morphology, and biochemical and immunological reactions. Appropriate references should be consulted for further information on identification procedures.
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Organisms Ref. No Result Legionella pneumophilia NCTC 11192 Growth, grey-white colonies Legionella anisa NCTC 11974 Growth, grey-white colonies Legionella bozemanae NCTC 11368 Growth, grey-white colonies Escherichia coli NCTC 12241 Inhibited Enterococcus faecalis NCTC 12697 Inhibited Pseudomonas aeruginosa NCTC 12903 Inhibited -
Listeria Oxford Selective Supplement E&O Laboratories Ltd Listeria Oxford Selective Supplement (LS0030) is a selective mixture used to supplement Listeria Oxford agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from clinical and food samples.
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Modified Campylobacter (Bolton) Selective Supplement E&O Laboratories Ltd Campylobacter Modified (Bolton) Selective Supplement (LS1054) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens.
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Neomycin Selective Supplement E&O Laboratories Ltd Neomycin Selective Supplement (LS0017) is an antibiotic supplement used to enhance the isolation of anaerobes from clinical specimens.
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Novobiocin Supplement (20mgs/L) E&O Laboratories Ltd Novobiocin Selective Supplement can be used to enhance the isolation of Salmonella spp. by motility enrichment and the selective enrichment of E.coli Serogroup O157 in food and faeces samples and also in the isolation of Salmonella typhimurium at 40mgs/L when used with Muller-Kauffmann Tetrathionate (MKTTn) Broth.
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Oxytetracycline (100mgs/L) Selective Supplement E&O Laboratories Ltd Oxytetracycline Selective Supplement can be used with a glucose, yeast or maltose extract based agar for selective isolation of Yeasts and Moulds from a variety of sample types. Due to the near neutral pH of the medium, Oxytetracycline is added to reduce the risk of bacterial growth when processing materials that may be more heavily contaminated. Media using oxytetracycline as the selective agent is based on the formulation developed by Mossel et al. who stated that the use of this antibiotic in a medium with a neutral pH gave increased counts of yeasts and moulds from a variety of foodstuffs compared with media which relied on a low pH to suppress bacterial growth.
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PALCAM Selective Supplement E&O Laboratories Ltd PALCAM Selective Supplement (LS0038) is a selective mixture used to supplement PALCAM agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from food samples.
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Primary Listeria Selective Supplement E&O Laboratories Ltd Primary Listeria Selective Supplement (LS0210) is a selective mixture used to supplement Primary Listeria Selective Agar (E&O PP7025) in order to facilitate the isolation of Listeria monocytogenes in foodstuffs and other samples.
