• GC Agar when used with blood and other enrichment is for the isolation of Neisseria gonorrhoeae but is also capable of supporting the growth of most fastidious micro-organisms. This medium is based on the modified formulation described by Thayer and Martin that was based on the original formulation stated by Johnson. Enrichment is usually attained using lysed blood but haemoglobin powder or chocolated blood are suitable alternatives. Additional enrichment can be provided by the addition of Suplex supplement (BM0478) which consists of yeast extract and glucose. Selective variants of GC Agar can be prepared through the addition of various selective supplements such as VCAT (LS0002) or LCAT (LS0001). These supplements will suppress most of the background flora likely to be present in specimen and will restrict the swarming of Proteus spp. The peptone is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Starch is present to absorb toxic metabolites and phosphate buffers prevent pH changes during incubation. Related Supplements : BM0478 Suplex, LS0001 GC LCAT Selective Supplement, LS0002 GC VCAT Selective Supplement
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  • R2A agar is used for the enumeration and cultivation of bacteria from drinking water. R2A agar developed by Reasoner and Geldreich is a low nutrient medium that can used in pour plate, spread plate, and membrane filtration methods for heterotrophic plate counts. In combination with a lower incubation temperature and longer incubation period R2A agar stimulates the growth of stressed and chlorine-tolerant bacteria. Traditionally nutritionally rich media have been used for this purpose but these media support the growth of fast-growing bacteria and may suppress slow growing or stressed bacteria found in treated water. Enzymatic digest of casein, proteose peptone, acid hydrolysate of casein and yeast extract provide the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. Dipotassium hydrogen phosphate is a buffering agent. Magnesium sulphate is a source of divalent cations and sulphate. Starch and sodium pyruvate aid in the recovery of stressed organisms.  
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  • Brain-heart infusion solids is a dehydrated infusion of porcine brains and heart. This product can be used in preparing microbiological culture media providing nitrogen, vitamins, minerals and amino acids.  
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  • Formulated to ISO 6887-1, Maximum Recovery Diluent (MRD) is a diluent designed to maintain organisms by protecting the cells from unnecessary physiological shock that may occur using other aqueous solutions. MRD is used extensively in food and environmental testing. The low level of peptone provides a protective effect but does not allow for multiplication during the short residence time in the diluent, 45 minutes. The sodium chloride prevents osmotic shock as the sample is initially diluted.  
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  • Peptone water is a general-purpose medium that supports thecultivation of non-fastidious organisms. This non-selective medium can be used a basal medium for biochemical tests such as carbohydrate fermentation and production of indole. Tryptone and peptone act as sources of carbon, nitrogen and vitamins in this medium and sodium chloride maintains osmotic balance.  
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  • Lactose broth is used for the performance and confirmation of the Presumptive Test for coliforms in water and dairy samples. This medium is also frequently used as a pre-enrichment medium when testing foods, water samples and dairy products for Salmonella spp. Beef extract and gelatin peptone provide the required carbon, nitrogen and vitamins in this medium. Lactose is a fermentable carbohydrate. Fermentation of lactose is detected by the production of gas
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  • Sabouraud Dextrose Agar is one of several media available for theisolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud Dextrose Agar is a modification of a medium originally described by Sabouraud. The tryptone and meat peptone provide the nitrogen and vitamins required for organism growth in Sabouraud Dextrose Agar. The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties. Higher levels of selectivity can be achieved against bacterial species by the addition of chloramphenicol. NB: This is a basic medium only and contains no additional supplements. Related Supplements : LS0050 Chloramphenicol Selective Supplement
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  • Plate Count Agar is formulated to the A.P.H.A. specification developed by Buchbinder et al. This medium is intended for use in food, dairy and water bacteriology to perform Total Viable Counts. Tryptone and yeast extract provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate.  
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