KM0029 – Tryptone Water

//KM0029 – Tryptone Water

KM0029 – Tryptone Water

Tryptone water is recommended for the detection of Escherichia coli in food and water samples. Tryptone water is an alternative medium to peptone water and is more reliable for the testing of indole production.
Tryptone acts as source of carbon, nitrogen and vitamins in this medium and sodium chloride maintains osmotic balance. The medium has a high content of tryptophan that many organisms, particularly coliforms, break down to form indole. After the recommended incubation indole positive organisms will give a distinct colour change when either Kovac’s or Ehrlich’s indole reagent is added.

SKU: KM0187 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Turbid growth, indole positive
Enterobacter aerogenes NCTC 10006 Turbid growth, indole negative

 

Formula*

g/L
Tryptone 10.0
Sodium chloride 5.0
Total 15.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 15g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and dispense into appropriate sterile containers.