Sabouraud dextrose agar with chloramphenicol is a selective medium for the isolation of yeasts and fungi suitable for use in all areas of mycology. This medium is a modification of that originally described by Sabouraud. The tryptone and meat peptone provide the required nitrogen and vitamins. The high concentration of dextrose provides an energy source for yeasts and fungi while, in tandem with the acidic pH (5.6), providing some selective properties. Chloramphenicol is included to increase the selectivity of the medium inhibiting a range of Gram-positive and Gram-negative bacteria.
KM0068 – Sabouraud Dextose Agar with Chloramphenicol
|Shelf Life (days)|
|Storage Temp (°C)|
|Dehydrated Medium Appearance|
|Prepared Medium Appearance|
|Dehydrated Medium Colour|
|Prepared Medium Colour|
Aerobically at 25-30°C for 2-7 days. Plates may be checked weekly for up to 5 weeks to confirm a specimen is culture negative.
|Saccharomyces cerevisiae||NCPF 3178||Growth|
|Aspergillus brasiliensis||NCPF 2275||Growth, white mycelia with black spores|
|Escherichia coli||NCTC 12241||Inhibited|
Suspend 65.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes.
Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.
NB: Avoid overheating this medium as the acidic pH may result in a softer final medium.