KM0068 – Sabouraud Dextose Agar with Chloramphenicol

//KM0068 – Sabouraud Dextose Agar with Chloramphenicol

KM0068 – Sabouraud Dextose Agar with Chloramphenicol

Sabouraud dextrose agar with chloramphenicol is a selective medium for the isolation of yeasts and fungi suitable for use in all areas of mycology. This medium is a modification of that originally described by Sabouraud. The tryptone and meat peptone provide the required nitrogen and vitamins. The high concentration of dextrose provides an energy source for yeasts and fungi while, in tandem with the acidic pH (5.6), providing some selective properties. Chloramphenicol is included to increase the selectivity of the medium inhibiting a range of Gram-positive and Gram-negative bacteria.

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Saccharomyces cerevisiaeNCPF 3178Growth
Aspergillus brasiliensisNCPF 2275Growth, white mycelia with black spores
Escherichia coliNCTC 12241Inhibited

Formula*

FORMULA*g/L
Tryptone5
Meat peptone5
Dextrose40
Agar15
Chloramphenicol0.05
Total65

Preparation

Suspend 65.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes.

Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.

 

NB: Avoid overheating this medium as the acidic pH may result in a softer final medium.