KM0165 – Fluid Thiogly Medium (USP)

//KM0165 – Fluid Thiogly Medium (USP)

KM0165 – Fluid Thiogly Medium (USP)

This medium is intended primarily for use in sterility testing of biological fluids that are turbid or viscous and is recommended in the United States Pharmacopoeia. It can be used for the cultivation of both aerobic and anaerobic organisms.

The yeast extract, tryptone and glucose act as carbon, nitrogen and vitamin sources in this medium. The inclusion of sodium thioglycollate and L-cystine ensure anaerobiosis even for many of the more fastidious anaerobes and they also inactivate mercury and many other heavy metallic compounds. Resazurin is an oxidation-reduction indicator which turns pink when oxidised and is colourless when reduced. Sodium chloride maintains osmotic balance. Agar reduces oxygen diffusion into the medium.

NB – For best results it is recommended that the medium be heated in a boiling water bath, with the cap loosened, and then allowed to cool, with the cap tightened, immediately before use. The cap must be replaced on the container immediately after inoculation.

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Bacteroides fragilisNCTC 9343Growth
Pseudomonas aeruginosaATCC 9027Growth
Staphylococcus aureusATCC 6538Growth
Clostridium perfringensNCTC 8237Growth
Aspergillus brasiliensisNCPF 2275Growth
Candida albicansNCPF 3179Growth

Formula*

FORMULA*g/L
L-cystine0.5
Sodium chloride2.5
Glucose5.5
Agar0.75
Yeast extract5
Tryptone15
Sodium thioglycollate0.5
Resazurin0.001
Total29.75

Preparation

Suspend 29.75g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes.