Each 500ml vial contains:
To reconstitute each vial aseptically add 2ml of Sterile Distilled Water. Close the vial, gently agitate to assist reconstitution. Avoid frothing and add the contents of the vial to 500ml of sterile base medium of choice prepared according to the manufactures instructions and allowed to cool to below 50°C. Mix gently but thoroughly and pour aseptically into sterile Petri dishes.
Overnight storage at 2-8°C is recommended to allow suitable equilibration of the supplement within the medium. For extended storage at 2-8°C e.g. plates should be contained in sealed plastic sleeves or similar packing.