This is a very nutritious general-purpose medium based on Columbia Agar Base enriched with 5% Horse Blood and β-Nicotinamide Adenine Dinucleotide (NAD). It is suitable for the isolation of most organisms, including Haemophilus influenzae, as well as many fastidious anaerobes of clinical significance.
PP0040 – Columbia Blood Base with 5% Defibrinated Horse Blood & NAD (40mg/L)
|Shelf Life (days)|
|Storage Temp (°C)|
Incubate H.influenzae & S.pneumoniae at 37°C ± 1°C in 5% CO2 for 18-24 hours., Incubate S.aureus & S.pyogenes at 37°C ± 1°C aerobically for 18-24 hours.
|Streptococcus pneumoniae||NCTC 12977||Green colonies with α-haemolysis|
|Staphylococcus aureus||NCTC 12981||White colonies|
|Haemophilus influenzae||NCTC 12699||Grey colonies|
|Streptococcus pyogenes||NCTC 12696||White colonieS with β-haemolysis|