PP0860 – Legionella MWY Agar

This is a selective medium for the isolation of Legionella spp used in clinical, water and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric pyrophosphate, L-Cysteine hydrochloride and α-Ketoglutarate. ACES buffer is incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Polymixin and Vancomycin to inhibit the majority of Gram-positive and Gram-negative organisms and Amphotericin B to inhibit yeasts and some fungi. Bromothymol blue and Bromocresol Purple are also included as an aid to identifying the organisms.