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E&O Laboratories Ltd Actinomycete Selective Supplement (LS0015) is an antibiotic supplement used to enhance the isolation of Actinomyces spp. -
Ampicillin Selective Supplement (100mgs/L) E&O Laboratories Ltd Ampicillin Selective Supplement can be used with LB Agar for selective cultivation of E. coli strains that contain plasmids conferring ampicillin resistance. Ampicillin is an antibiotic used to treat a number of bacterial infections. It is a beta-lactam antibiotic that is part of the amino penicillin family. It is active against many Gram-positive and Gram-negative bacteria.
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B.cepacia Selective Supplement E&O Laboratories Ltd Burkholderia Selective Supplement (LS0125) is an antibiotic supplement used to enhance the isolation of Burkholderia cepacia (formerly known as Pseudomonas cepacia).
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Bacillus cereus Selective Supplement E&O Laboratories Ltd Bacillus Cereus Selective Supplement (LS0020) is an antibiotic supplement used to enhance the selective isolation of Bacillus cereus from foodstuffs and clinical specimens.
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Bacitracin Selective Supplement E&O Laboratories Ltd Bacitracin Supplement (LS0012) is an antibiotic supplement used to enhance the isolation of Haemophilus species.
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Bordetella Selective Supplement Bordetella Selective Supplement LS0018 is an antibiotic supplement used to enhance the culture of Bordetella pertussis.
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Camplobacter (CCDA) Selective Supplement E&O Laboratories Ltd Campylobacter (CCDA) Selective Supplement (LS0010) is an antibiotic supplement used to enhance the selective isolation of Campylobacter spp. from clinical, food and environmental samples.
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Campylobacter (Skirrow) Selective Supplement E&O Laboratories Ltd Campylobacter (Skirrow) Selective Supplement (LS0009) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens.
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Campylobacter Growth Supplement E&O Laboratories Ltd Campylobacter Growth Supplement (LS0011) is a supplement used to enhance the isolation and aerotolerance of Campylobacter spp and can be used in both solid and liquid media and generally used in conjunction with media prepared to Skirrow, Butzler or Preston formulations.
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Cefotaxime Selective Supplement E&O Laboratories Ltd Cefotaxime Selective Supplement can be used with MacConkey Agar for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing organisms with the aim of simplifying the differentiation and presumptive identification of Lactose & Non-Lactose fermenting organisms. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. Sodium Chloride is omitted from this medium to provide an electrolyte deficient medium that prevents Proteus spp. from spreading.
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This medium is based on the formulation published by Platt, Atherton & Simpson1 and is used for the cultivation of Taylorella equigenitalis, the causative organism in contagious equine metritis. It is routinely used for culturing swabs taken from the genitalia of mares and stallions. Enzymatic digest of casein and soy peptone supply nitrogen, carbon and vitamins and L-cystine is a required growth factor. Sodium chloride provides osmotic balance and sodium sulphite is present as a reducing agent. The medium is made selective by the addition of CEMO Selective Supplement (LS0041) to control bacteria and fungi from swab samples. References 1. Platt, H., Atherton, J. G. and Simpson, D. J. 1978. Equine Vet J 10, 153–159. -
Chloramphenicol Selective Supplement (100mgs/L) E&O Laboratories Limited Chloramphenicol Selective Supplement (LS0050) is an antibiotic supplement used for the selective isolation of yeasts and moulds from food, environmental and clinical specimens.
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Clostridium difficile Selective Supplement E&O Laboratories Ltd Clostridium Difficile Selective Supplement (LS0022) is an antibiotic supplement used to enhance the isolation of Clostridium difficile from faecal specimens.
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Escherichia coli 0157 Selective Supplement E&O Laboratories Ltd Escherichia coli 0157 Selective Supplement (LS0013) is an antibiotic supplement used to enhance the isolation of Escherichia coli serogroup O157 from faecal, food and environmental specimens.
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Ferric Ammonium Citrate (FAC) Supplement E&O Laboratories Ltd Ferric Ammonium Citrate (LS5004) is a supplement used in the isolation of Listeria spp. with Fraser Broth. Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. The medium is intended for the isolation of Listeria spp. from food and environmental samples when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. It is generally accepted that the USDA-FSIS two stage enrichment method employing UVM primary and secondary enrichment broths is the most suitable for the examination of meat products.
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Fraser Broth Selective Supplement E&O Laboratories Ltd Fraser Broth Selective Supplement (LS5002) is an antibiotic supplement used to enhance the selective isolation of Listeria spp. in combination with Fraser Broth.
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Vancomycin. Colistin, Amphotericin, Trimehtoprim (V.C.A.T) E&O Laboratories Ltd VCAT Selective Supplement (LS0002) is an antibiotic supplement used to enhance the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.
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GC LCAT Selective Supplement E&O Laboratories Ltd LCAT Selective Supplement (LS0001) is an antibiotic supplement used to enhance the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.
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Half Fraser Broth Selective Supplement E&O Laboratories Ltd Half Fraser Selective Supplement (LS5003) is selective supplement for the primary enrichment of Listeria spp from food and environmental samples when used with Fraser Broth.Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. It contains ferric ammonium citrate and lithium chloride. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. Fraser Broth has proven to be remarkably accurate in detecting Listeria spp. in food and environmental samples. All Listeria spp. hydrolyse aesculin to aesculetin. Aesculetin reacts with ferric ions which results in blackening. Another possible advantage to the addition of ferric ammonium citrate is that it has been shown that ferric ions enhance the growth of Listeria monocytogenes. Lithium chloride is included in the medium to inhibit the growth of enterococci which can also hydrolyse aesculin. Half Fraser Broth is a modification of Fraser Broth which contains half of the concentration of nalidixic acid and acriflavine hydrochloride to aid in the recovery of stressed cells. Half Fraser Broth is used as the primary enrichment broth in the ISO methodology for the detection of Listeria.
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Helicobacter pylori Selective Supplement E&O Laboratories Ltd Helicobacter pylori Selective Supplement can be used with Helicobacter pylori Base Agar (E&O product KM0009).This selective supplement is composed of 2 antibiotics and one antifungal agent. These agents inhibit the background flora and allow the cultivation of Helicobacter pylori. Helicobacter pylori, previously named Campylobacter pylori, is a Gram-negative, microaerophilic bacterium found in the stomach, and may be present in other parts of the body, such as the eye.
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Kanamycin Selective Supplement (50mgs/L) E&O Laboratories Ltd Kanamycin Selective Supplement can be used with LB Agar for selective cultivation of E. coli and other organisms that contain plasmids conferring kanamycin resistance. Kanamycin is an aminoglycoside bacteriocidal antibiotic used to treat a wide variety of infections Kanamycin is isolated from the soil bacterium Streptomyces kanamyceticus.
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Listeria Oxford Selective Supplement E&O Laboratories Ltd Listeria Oxford Selective Supplement (LS0030) is a selective mixture used to supplement Listeria Oxford agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from clinical and food samples.
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Modified Campylobacter (Bolton) Selective Supplement E&O Laboratories Ltd Campylobacter Modified (Bolton) Selective Supplement (LS1054) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens.
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MSS is a viral transport solution designed to facilitate collection and transport of viruses safely from site of sampling to a laboratory location at ambient temperatures. The medium is buffered to help maintain a pH of 7.1, and contains a metal ion chelator, a non-ionic surfactant and a chaotropic agent to ensure disruption of virus particles, denaturation of viral proteins and inactivation of nucleases that may destroy target nucleic acids. The resultant sample is suitable for virus identification by PCR analysis as well as other nucleic acid manipulations. MSS is designed to disrupt viral particles, denature viral proteins and, therefore, reduce the infection hazard during transport and laboratory processing. NB – BM1677 MSS has been validated only for use with the SARS-CoV-2 virus 2ml in self-standing M043 Tube • Aseptically filled • Red cap with swab capture point • Storage: 2-30°C • Store in the dark • Shelf life: 730 days • Samples collected in MSS are suitable for PCR analysis and other nucleic acid manipulations. • MSS is designed to disrupt viral particles, denature viral proteins, inactivate nucleases and reduce the infection hazard during transport and laboratory processing. -
The MSS (Molecular Sampling Solution) Swab Kit is designed to facilitate collection, inactivation and transport of patient specimens safely from the site of sampling to a laboratory location. The kit consists of a versatile sterile swab* for specimen collection and a self-standing polypropylene tube with swab capture cap containing Molecular Sampling Solution (MSS) inactivating transport medium. MSS is designed to disrupt microorganisms (including viruses and bacteria), release nucleic acids, denature proteins, and destroy nucleases. This reduces the infection hazard during transport and laboratory processing, as well as preserving nucleic acids for identification by PCR or other nucleic acid techniques. The stability of nucleic acids afforded by the MSS enables transport and storage in the 2-30°C range**. Viral nucleic acids remain stable in MSS at room temperature for at least 14 days. MSS (Molecular Sampling Solution) The MSS formulation is buffered to help maintain pH and contains a buffering agent (Tris), a metal ion chelator (EDTA), a non-ionic surfactant, and a chaotropic agent (guanidine isothiocyanate) to ensure disruption of microorganisms, denaturation of proteins, release of nucleic acids, and inactivation of nucleases that may destroy target nucleic acids. The resultant sample is suitable for target identification by PCR analysis as well as other nucleic acid techniques. SW0004 MSS Swab Kit cannot be used for cell culture-based diagnostic testing. *Not suitable for nasopharyngeal swabbing. **Must be stored in the dark. -
Neomycin Selective Supplement E&O Laboratories Ltd Neomycin Selective Supplement (LS0017) is an antibiotic supplement used to enhance the isolation of anaerobes from clinical specimens.
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Novobiocin Supplement (20mgs/L) E&O Laboratories Ltd Novobiocin Selective Supplement can be used to enhance the isolation of Salmonella spp. by motility enrichment and the selective enrichment of E.coli Serogroup O157 in food and faeces samples and also in the isolation of Salmonella typhimurium at 40mgs/L when used with Muller-Kauffmann Tetrathionate (MKTTn) Broth.
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Oxytetracycline (100mgs/L) Selective Supplement E&O Laboratories Ltd Oxytetracycline Selective Supplement can be used with a glucose, yeast or maltose extract based agar for selective isolation of Yeasts and Moulds from a variety of sample types. Due to the near neutral pH of the medium, Oxytetracycline is added to reduce the risk of bacterial growth when processing materials that may be more heavily contaminated. Media using oxytetracycline as the selective agent is based on the formulation developed by Mossel et al. who stated that the use of this antibiotic in a medium with a neutral pH gave increased counts of yeasts and moulds from a variety of foodstuffs compared with media which relied on a low pH to suppress bacterial growth.
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PALCAM Selective Supplement E&O Laboratories Ltd PALCAM Selective Supplement (LS0038) is a selective mixture used to supplement PALCAM agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from food samples.
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Primary Listeria Selective Supplement E&O Laboratories Ltd Primary Listeria Selective Supplement (LS0210) is a selective mixture used to supplement Primary Listeria Selective Agar (E&O PP7025) in order to facilitate the isolation of Listeria monocytogenes in foodstuffs and other samples.
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Pseudomonas CFC Selective Supplement E&O Laboratories Ltd Pseudomonas CFC Selective Supplement (LS0026) is an antibiotic supplement used to enhance the selective isolation of pseudomonads primarily from food and environmental samples.
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E&O Laboratories Limited Pseudomonas Selective CN Supplement (LS0006) is an antibiotic supplement used to enhance the selective isolation of Pseudomonas species particularly Pseudomonas aeruginosa.
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This is a variation on sterile isotonic Saline which is suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. The addition of glass beads will allow dense material to be broken down and aid the isolation of any bacteria that may be present in “clumps”. This particular product is double wrapped and terminally sterilised by Ethylene oxide. -
Staph/Strep Selective Supplement E&O Laboratories Ltd Staph/Strep Selective Supplement (LS0008) is an antibiotic supplement used to enhance the selective isolation of Staphylococci and Streptococci species.
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Tryptose is a mixed enzymatically digested protein. This product can be used in preparing microbiological culture media providing unique nutritional properties useful for cultivating fastidious microorganisms.
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b-Nicotinamide-Adenine Dinucleotide (N.A.D.) Supplement E&O Laboratories Ltd N.A.D. Supplement (LS0005) is an enrichment supplement for Haemophilus spp. and Neisseria gonorrhoeae. This supplement can be used in conjunction with sensitivity test agars such as: E&O products PP2148 Iso Sensitivity test agar with 5% Horse Blood and 20mgs/L N.A.D. and PP0972 Mueller Hinton agar with 5 % Horse Blood with 20mgs/L N.A.D.
