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Phosphate Buffered Saline (PBS) with Beads is an isotonic diluent for the maximum recovery of mycobacteria. The low nutritional content of the diluent prevents organism multiplication in the sample for a minimum of one hour. Sodium chloride maintains the osmotic balance and the beads help to separate mycobacteria cell clumps. -
E&O Laboratories Ltd Clostridium perfringens (TSC) Selective Supplement (LS5005) is an antibiotic supplement used to enhance the selective isolation of Clostridium perfringens from clinical specimens and foodstuffs. -
E&O Laboratories Ltd Legionella Growth Supplement (LS0053) is an enrichment supplement used to enhance the isolation of Legionella spp. in combination with Charcoal Yeast Extract (CYE) agar. CYE Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of containing the bacteria.
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E&O Laboratories Ltd Legionella BMPA Supplement (LS0156) is used in the identification of suspected colonies of Legionella spp. when used to prepare Legionella BMPA agar. Legionella BMPA Supplement contains cefamandole and polymyxin B to inhibit most Gram-positive and Gram-negative organisms.
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E&O Laboratories Ltd Legionella GVPC Selective Supplement (LS0052) is used in the culture of suspected colonies of Legionella spp. Colonies of Legionella should be visible after 2 to 5 days incubation on BCYE agar with growth supplement and appear light blue to blue-grey in colour. Colonies suspected of being Legionella should be Gram-stained and subcultured to a fresh Legionella agar plate and to a Legionella agar plate not containing L-Cysteine. Gram-negative organisms that grow on Legionella Agar but fail to grow on Legionella agar (without L-Cysteine) may be presumptively identified as Legionella species. Definitive identification is performed on the basis of growth, morphology, and biochemical and immunological reactions. Appropriate references should be consulted for further information on identification procedures.
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Organisms Ref. No Result Legionella pneumophilia NCTC 11192 Growth, grey-white colonies Legionella anisa NCTC 11974 Growth, grey-white colonies Legionella bozemanae NCTC 11368 Growth, grey-white colonies Escherichia coli NCTC 12241 Inhibited Enterococcus faecalis NCTC 12697 Inhibited Pseudomonas aeruginosa NCTC 12903 Inhibited -
E&O Laboratories Ltd Yersinia (C.I.N.) Selective Supplement (LS1053) is an antibiotic supplement used to enhance the selective isolation of Yersinia spp. in combination with Yersinia C.I.N. Agar.
