As requested

//As requested
  • This is best described as a multi-purpose medium for differentiation of enterobacteriacae that combines three individual tests into a single medium. For use the medium is inoculated by making a single stab into the medium with a straight wire (or equivalent) using a pure culture (or discrete single colony) of the test organism. Following incubation it is recommended that the medium should first of all be examined to determine whether or not the organism is motile. The presence of motility is apparent by the organism tracking out from the line of inoculation and often turning the medium turbid. Non-motile organisms generally grow within the stab line leaving the surrounding medium clear. Urease positive organisms (e.g. Proteus spp) turn the medium bright red due to the hydrolysis of the Urea in the presence of the Phenol Red Indicator often making it difficult to determine the other parameters.Indole is tested for by layering a small amount of Indole Reagent (Erlich’s or Kovac’s appear to work equally well) onto the surface of the medium and allowed a few minutes to react. A positive result is indicated by the formation of a red line at the interface of the reagent and the medium.
  • This medium is recommended for the detection and enumeration of yeasts and moulds in food and dairy products. It can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. The low pH (5.6) suppresses the growth of most bacteria and the low mineral content ensures good pigment production by fungi where appropriate.
  • BM0274

    R2A Agar

    Traditionally, standard methods for enumeration of heterotrophic bacteria in water have used nutritionally rich media with incubation at 35°C. This may represent only a small percentage of bacteria present in the sample. R2A agar is a nutritionally reduced medium which, when combined with incubation at lower temperatures for longer time periods, results in enhanced recovery of stressed and chlorine damaged organisms from treated waters resulting in higher more realistic bacterial counts.
  • A medium for the selective enrichment of Salmonellae spp from both clinical and food samples. It is a buffered Lactose Peptone Broth to which Sodium Biselenite is added as the selective agent and Cystine to enhance the recovery of salmonella in low numbers. Subcultures should be made from the top 1/3 of the broth after not more than 24 hours incubation as after this time there is a loss of selectivity.
  • This is a sterile aqueous solution of Potassium Di-Hydrogen Ortho-phosphate (16.0 % w/v) suitable for use in the neutralisation following the digestion of Sputum with Sodium Hydroxide prior to culture. It is generally used in conjunction with Sodium Hydroxide 4% with Phenol Red Indicator 0.2%.
  • A nutritious broth medium formulated by Todd and Hewitt for the production of antigenic streptococcal haemolysin. The broth is also used to cultivate streptococci prior to serological grouping. Normally the use of fermentable sugars in the broth would inactivate the haemolysin due to acid production. This is prevented by the use of buffers to maintain the pH in this broth.
  • This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds.  The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B).  Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation.
  • BM0460

    Urea Broth

    A modification of Christensen’s Medium by Maslen this medium is generally used to detect rapid Urease activity of Proteus spp although it can be used to detect Urease activity of other Enterobacteriaceae including Urease producing Salmonella and Shigella. Unlike Christensen’s Medium when used for the later purpose it is not necessary to increase the incubation time.