-
Alkaline Peptone Water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. The high pH of the medium inhibits most enteric organisms for at least 24 hours. The medium is heavily inoculated with faeces and after not more than 8 hours incubation a loopful from the top of the medium is sub cultured onto TCBS Agar. This enrichment medium is also used for food and water testing. -
An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms. -
An enriched general purpose broth being an isotonic medium with Tryptose providing a wide range of substrates. The medium is further enriched with 10% horse serum for more fastidious organisms and as an enrichment broth. -
BM0070 Brain Heart Infusion Broth is a nutritious, isotonic, general-purpose medium suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. Modern BHI typically uses an infusion from porcine brains and hearts rather than calf brain tissue, and uses disodium phosphate as a buffer, rather than the calcium carbonate used by Rosenow and Haden. It is a nutrient-rich medium and can therefore be used to culture a variety of fastidious organisms, including streptococci, pneumococci, and meningococci, which can be challenging to grow. Brain Heart Infusion Broth is recommended by the FDA BAM for the enrichment for pathogenic Escherichia coli in foods and environmental samples, and as a medium used in the coagulase test for the identification of Staphylococcus aureus from foods, and Gram-positive cocci from cosmetic samples. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture. -
This medium is used to detect and/or confirm the presence of coli-aerogenes group of organisms in water, food and dairy laboratories. Bile and Brilliant Green are included in the medium to inhibit gram positive organisms while the coli-aerogenes organisms are identified by the formation of gas during the fermentation of Lactose. The medium can also be used for the confirmation of Escherichia coli by incubating at 44°C. -
Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms. -
A pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective medium. This non-selective, nutritious medium is free from inhibitors and is well buffered to maintain the pH at 7.0 for the incubation period according to ISO 6579 (2002). -
Buffered Sodium Chloride Peptone Diluent is used for the microbial examination of pharmaceutical products, e.g. as a diluent for sample preparation or as a rinsing solution. This formulation complies with the Harmonized USP/EP/JP.
