BM0160 Fastidious Anaerobe Broth is designed for the culture of fastidious anaerobes, particularly Bacteroides species, and is also suitable for use as an anaerobic blood culture medium. BM0160 is recommended by the UK Standards for Microbiology Investigations and is formulated and tested in compliance with the principals of ISO 11133:2014.
Peptone and yeast extract supply the required carbon, nitrogen, and vitamins. Haemin, vitamin K, L-arginine, and L-cysteine HCl are additional growth factors required by some anaerobes. Sodium thioglycolate and L-cysteine HCl help lower the pH of the medium to maintain anaerobicity and starch and sodium hydrogen carbonate help neutralise toxins. The increased viscosity produced by addition of agar helps prevent oxygen diffusion in the final medium. Resazurin is a redox indicator and sodium chloride maintains the osmotic balance.
BM0160 Fastidious Anaerobe Broth is designed for the culture of fastidious anaerobes, particularly Bacteroides species, and is also suitable for use as an anaerobic blood culture medium. BM0160 is recommended by the UK Standards for Microbiology Investigations and is formulated and tested in compliance with the principals of ISO 11133:2014. Peptone and yeast extract supply the required carbon, nitrogen, and vitamins. Haemin, vitamin K, L-arginine, and L-cysteine HCl are additional growth factors required by some anaerobes. Sodium thioglycolate and L-cysteine HCl help lower the pH of the medium to maintain anaerobicity and starch and sodium hydrogen carbonate help neutralise toxins. The increased viscosity produced by addition of agar helps prevent oxygen diffusion in the final medium. Resazurin is a redox indicator and sodium chloride maintains the osmotic balance.
A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. The addition of glass beads will allow for the maceration of the sample and aid the homogenisation of the suspension.
