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This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. Designed for use with NaOH treated samples which have sterile distilled water added at the end of the designated treatment time to "dilute" the effect of NaOH. NB: this media will NOT isolate M.bovis which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium. -
This is a very nutritious general-purpose medium suitable for the isolation of most organisms including many fastidious anaerobes. It is particularly recommended for streptococci and neisseria. -
A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. The addition of glass beads will allow for the maceration of the sample and aid the homogenisation of the suspension. -
Kirchner medium is a liquid medium for the selective enrichment and isolation of Mycobacteria spp from clinical specimens, particularly when the organisms may be present only in small numbers (e.g. CSF and tissue biopsies). This medium has been enriched by the addition of calf serum as it is a widely used supplement because it is rich in growth factors. The medium is made selective by the inclusion of Polymyxin B, Ticarcillin and Trimethoprim to inhibit other bacteria and Amphotericin B to inhibit yeasts and fungi. It is generally used in conjunction with a solid medium such as Lowenstein Jensen Medium. Specimens from normally sterile body sites may be inoculated without digestion and decontamination. Other specimens should be pre-treated according to standard procedures. The inoculated medium should be incubated at 35-37°C for up to 8 weeks. Kirchner medium should be used in conjunction with a solid medium (e.g. Lowenstein-Jensen medium) in order to accelerate differentiation and identification testing.
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This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis, from clinical samples. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein Jensen Medium slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium
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This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. Designed for use with NaOH treated samples which are neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto LJ media after concentration by centrifugation. NB: this media will NOT isolate M.bovis which requires the addition of pyruvate as a growth supplement.This is normally overcome by the inclusion of a pyruvate slope. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
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This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp. Designed for use with NaOH treated samples which are neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto LJ media after concentration by centrifugation. This medium will isolate most common mycobacteria including M.bovis. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
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This is a variation on the common osmotically controlled Ringers solution, where glass beads have been added to enhance macerations in the preparation of suspensions of samples and for use as a diluent in dilution techniques for bacterial enumeration. The solution may also be used in the sampling of food production apparatus by the rinse and swab method. -
This is a variation on sterile isotonic Saline which is suitable for use in preparation of food samples and/or as a rinse during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory. The addition of glass beads will allow dense material to be broken down and aid the isolation of any bacteria that may be present in “clumps”. This particular product is double wrapped and terminally sterilised by Ethylene oxide.
