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This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia (USP) for antibiotic sensitivity testing of pharmaceutical products. As previously stated Antibiotic Medium No. 1 is used in the performance of antibiotic assays. This medium is prepared according to the specifications detailed in the USP. The use of this medium assures well-defined inhibition zones of the test organisms. Nutrients and growth factors are supplied by peptic digest of animal tissue, casein hydrolysate, yeast extract, and beef extract. Glucose is a carbon source. -
This is a medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for antibiotic sensitivity testing of pharmaceutical products. Antibiotic Assay Medium No. 8 is recommended for preparing inoculum of Bacillus subtilis to be used as a test for assaying Vancomycin by plate assay method. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. Fluid D has been formulated with the addition of polysorbate 80 to enable the rinsing of products containing lecithin or oil. This formulation complies with the Harmonised USP/EP/JP. -
de Man, Rogosa & Sharpe (MRS) Agar M.R.S. Agar is intended for the cultivation and enumeration of Lactobacillus spp from a variety of sources and can be used as an alternative to Orange Serum Agar for that purpose. Magnesium Sulphate, Manganese Sulphate, Sodium Acetate and Tween are included as growth supplements. The medium can be made more specific for lactobacilli generally by lowering the pH to between 5.0 and 5.5. This has the effect of inhibiting most streptococci that may otherwise grow on the medium and can be readily confused with lactobacilli. -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed primarily as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available.
