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This is a 25ml fill selective medium for the isolation of Actinomyces spp from clinical specimens. Based on Fastidious Anaerobe Agar enriched with 7% Horse Blood, the medium has been made selective by the inclusion of Nalidixic Acid to inhibit most aerobes, particularly gram-negative bacilli, and Metronidazole to suppress other anaerobes. -
Bacillius cereus Selective Agar (PEMBA) This is a medium for the selective isolation and enumeration of Bacillus cereus in food samples. It is said to be particularly suitable for the detection of small numbers of Bacillus cereus particularly in the presence of other contaminating organisms. The medium is made selective by the inclusion of Polymixin and Sodium Pyruvate is also present which is said to improve Egg Yolk precipitation and enhance sporulation. As Bacillus cereus is Mannitol Negative the colonies are bluish in colour, due to the presence of the Bromothymol Blue Indicator, with a surrounding precipitate of the same colour due to Lecithinase production (from the Egg Yolk). NB: It should be noted that some Proteus spp. and gram positive cocci may grow on this medium. -
Bile Aesculin Agar Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc. -
A general-purpose medium enriched with 7% Horse Blood, suitable for the isolation of most organisms including many fastidious anaerobes of clinical significance. -
A general purpose medium enriched with 5% Defibrinated Sheep Blood, suitable for the isolation of most organisms including many fastidious anaerobes. -
Brazier's CCEY Agar with 1% Lysed Horse Blood - Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light. -
Brilliant Green Agar This medium is intended for use in the isolation of Salmonellae other than Salmonella typhi. Although it can be used as a primary isolation medium it is not recommended for this purpose and is generally used for subculture from selective enrichment media. It should be noted that the medium is highly selective and therefore not suited to the isolation of Salmonella from samples where the numbers may be low. NB: It is not recommended that this medium be used for the isolation of Salmonella typhi and Shigella spp. -
This is a selective medium for the isolation of Burkholderia cepacia. The base contains Bile Salts and Crystal Violet as selective agents and Ticarcillin and Polymixin B are added as additional supplements to further improve the selectivity particularly inhibition of most Pseudomonas spp. -
PP0130 Campylobacter Blood Free CCDA is designed for the selective isolation of Campylobacter spp., particularly C. jejuni and C. coli, which are major causes of gastrointestinal infection. Campylobacter Blood Free CCDA was described by Bolton et al. and formulated to replace blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate. This product is recommended for the isolation of Campylobacter spp. and complies with the requirements of ISO 10272-1:2017, ISO 10272-2:2017, is tested in accordance with ISO 11133:2014, and is a recommended medium in the UK Standards for Microbiology Investigations for isolation of Campylobacter spp. from clinical specimens such as faeces. -
Campylobacter Selective Agar Preston Supplement This is one of several selective media available for the isolation of Campylobacter spp in clinical, food and environmental laboratories. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Cefoperazone, to suppress other enteric organisms, and Amphotericin to suppress yeast & fungal growth. -
Campylobacter Selective (Skirrow) Agar This is one of several selective media available for the isolation of Campylobacter spp in clinical, food and environmental laboratories. Based on Columbia Agar enriched with Lysed Horse Blood. Polymyxin B, Trimethoprim & Vancomycin are added as the selective agents. Sodium Thiosulphate, Pyruvic Acid and Ferrous Sulphate are also included to enhance the aerotolerance of Campylobacter spp. NB: This medium should be incubated at 42°C to optimise selectivity. -
This is a medium intended for the cultivation and isolation of Bordetella pertussis & Haemophilus spp. The base medium contains Charcoal and is enriched with 10% Horse Blood. It can also be used as a maintenance or transport medium for these organisms. -
Charcoal Agar with 10% Horse Blood & Cephalexin This is one of two media generally used for the selective isolation of Bordetella pertussis. The medium is made selective by the inclusion of Cephalexin, to suppress the unwanted naso-pharyngeal flora often present in specimens submitted for the isolation of Bordetella pertussis, and further enriched with 10% Horse Blood. NB: Although coliforms are inhibited by this medium, Pseudomonas aeruginosa and some fungi will grow. -
A highly nutritious medium used for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophillus species from a variety of clinical specimens. The media is further enriched with Suplex (Polyvitex) that provides vitamins, amino acids, co-enzymes, glucose and other factors which improve the growth of Neisseria and Haemophillus species. -
A highly nutritious medium enriched with Horse Blood, where the blood has been “chocolated” by heating the medium to 60°C. Suitable for the isolation of most pathogens including the most fastidious and is particularly useful for the cultivation of Haemophilus spp. and Neisseria spp. -
CLED Agar Mackey and Sandy’s formulation this medium is popular for Urine Culture in the clinical laboratory. The lack of electrolytes inhibits the spreading of Proteus spp. and Bromothymol Blue indicator allows easy differentiation of Lactose and Non-Lactose fermenting organisms. Cystine is also present to benefit those organisms that have a particular Cystine requirement. Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium.
