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Brazier's CCEY Agar with 1% Lysed Horse Blood - Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light. -
Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms. -
A pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective medium. This non-selective, nutritious medium is free from inhibitors and is well buffered to maintain the pH at 7.0 for the incubation period according to ISO 6579 (2002). -
Buffered Sodium Chloride Peptone Diluent is used for the microbial examination of pharmaceutical products, e.g. as a diluent for sample preparation or as a rinsing solution. This formulation complies with the Harmonized USP/EP/JP. -
Colorex™Acinetobacter MDR is a chromogenic medium for the detection of multi-drug resistant (MDR) Acinetobacter spp. Positive colonies exhibit a distinct red colouration with a pale grey centre enabling easy interpretation amongst blue, violet or colourless colonies that may be produced by other Gram –ve bacteria. Gram +ve bacteria and yeast are inhibited on this medium. Limitations: 1. Some Pseudomonas, Stenotrophomonas and Burkholderia spp. may form pale red colonies on this medium but are readily distinguishable due to differences in colonial morphology compared to the Acinetobacter spp. An oxidase test will readily differentiate any Pseudomonas spp. 2. Some Enterobacteriaceae isolates may form blue colonies on this medium. 3. Definitive MDR characterisation may require additional antibiotic susceptibility testing. -
Colorex C3GR is a chromogenic screening medium for the detection of β-Lactamase producing Gram-negative bacteria in clinical specimens. The selectivity of the medium allows for detection of ESBL and/or AmpC producing isolates that exhibit a reduced susceptibility to 3rd generation cephalosporin antibiotics. The chromogenic reactions allow for species differentiation on presumptive positive isolates. -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the Enterobacteriaceae. Colorex™ ESBL (Extended Spectrum Beta-Lactamase) medium has been developed for the isolation of ESBL – producing organisms with the aim of simplifying the differentiation and presumptive identification of the causative organism. It should be noted that other non-ESBL and AmpC isolates will be inhibited on this medium reducing the incidence of false positives. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium. Typical colour reactions are as follows: Escherichia coli – Red colonies; Proteus spp., Providencia spp. & Morganella spp. – Clear colonies with a brown halo; Klebsiella spp., Enterobacter spp, Serratia spp. & Citrobacter spp. – Metallic blue colonies; Salmonella spp. & Acinetobacter spp. – Clear colonies; Gram +ve bacterial species and yeasts – Inhibited. -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. Colorex™ UTI has been developed primarily for use in the examination of urine specimens with the aim of simplifying the differentiation and presumptive identification of the main organisms, gram negative and gram positive, usually found in Urinary Tract Infections. It can however be used to differentiate organisms in other types of clinical specimens. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium.
