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Brilliant green agar is for the selective isolation of Salmonella spp., other than S. typhi. Brilliant green agar was first cited by Kristensen et al. in 1925 but was subsequently modified by the Netherlands Institute for Public Health. Brilliant green agar is generally used in conjunction with XLD agar (KM0013) as a secondary plating medium for subculture from selective enrichment media in food and environmental testing. It should be noted that the medium is highly selective and therefore not suited to the isolation of Salmonella spp. from samples where the numbers may be low. Beef extract, peptone and yeast extract provide the required carbon, nitrogen and vitamins. Di-sodium hydrogen phosphate and sodium di-hydrogen phosphate are buffering agents. Lactose and sucrose are fermentable carbohydrates. Phenol red is a pH indicator and turns the medium yellow during lactose and/or sucrose fermentation. Brilliant green inhibits Gram-positive bacteria and Gram-negative bacilli other than Salmonella spp. NB: It is not recommended that this medium be used for the isolation of Salmonella typhi or Shigella spp. Related Supplements : LS0024 Novobiocin Supplement (20mgs/L)
