Bile Aesculin Agar

Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.

Additional Information


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Shelf Life (days)

Storage Temp (°C)





Product Description

Organisms Ref. No Result
Enterococcus faecalis NCTC 12697 Grey colonies with blackening of the media.
Streptococcus pyogenes NCTC 12696 Inhibited


Incubate aerobically at 37 ± 1°C for 18-24 hours.