This is best described as a multi-purpose medium for differentiation of enterobacteriacae that combines three individual tests into a single medium. For use the medium is inoculated by making a single stab into the medium with a straight wire (or equivalent) using a pure culture (or discrete single colony) of the test organism. Following incubation it is recommended that the medium should first of all be examined to determine whether or not the organism is motile. The presence of motility is apparent by the organism tracking out from the line of inoculation and often turning the medium turbid. Non-motile organisms generally grow within the stab line leaving the surrounding medium clear. Urease positive organisms (e.g. Proteus spp) turn the medium bright red due to the hydrolysis of the Urea in the presence of the Phenol Red Indicator often making it difficult to determine the other parameters.Indole is tested for by layering a small amount of Indole Reagent (Erlich’s or Kovac’s appear to work equally well) onto the surface of the medium and allowed a few minutes to react. A positive result is indicated by the formation of a red line at the interface of the reagent and the medium.
|Shelf Life (days)|
|Storage Temp (°C)|
|Escherichia coli||NCTC 12241||Urease -ve/ motility +ve / indole +ve|
|Proteus mirabilis||NCTC 10975||Urease +ve / motility +ve / indole -ve|
|Klebsiella pneumoniae||NCTC 9633||Urease -ve / motility -ve / indole -ve|
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