Non-acidified Glycerol Lowenstein Jensen Medium

///Non-acidified Glycerol Lowenstein Jensen Medium

Non-acidified Glycerol Lowenstein Jensen Medium

BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis.

BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is designed for use with sodium hydroxide (NaOH) treated specimens that have been neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto the LJ medium after concentration by centrifugation.

This medium will isolate most common mycobacteria including M. tuberculosis and can be used for the presumptive identification of mycobacterial infections, testing antibiotic susceptibility of isolates, and differentiating different species of Mycobacterium (by colony morphology, growth rate, biochemical characteristics, and microscopy).

BM8200 is recommended by the UK Standards for Microbiology Investigations.

NB: This media will not isolate wild strains of M. bovis, which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope such as E&O BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium.

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Organisms Ref. No Result
Mycobacterium tuberculosis ATCC 35818 Growth: Cream colonies with a rough surface
Mycobacterium bovis NCTC 5692 Growth: Cream colonies with a rough surface
Mycobacterium kansasii NCTC 13024 Growth: Cream colonies with a rough surface
Mycobacterium fortuitum NCTC 10394 Growth: Cream colonies with a rough surface
Mycobacterium intracellulare NCTC 10682 Growth: Cream colonies with a rough surface