Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes including fastidious organisms. The formulation is complex and includes detoxification agents (starch & sodium bicarbonate) as well as growth enhancing agents (cysteine, arginine, vitamin K, sodium succinate, glucose and pyrophosphate) as well as haemin to encourage pigment production in Porphyromonas melaninogenicus . Sodium pyruvate is also included to help neutralise hydrogen peroxide. In this instance the medium is made more selective by the inclusion of neomycin and aztreonam to inhibit most enteric organisms and further enriched by the addition of 7% horse blood.
Fastidious Anaerobe Agar with 7% Horse Blood, Neomycin (75mg/L) & Aztreonam (2mg/L)
|Shelf Life (days)|
|Storage Temp (°C)|
|Bacteroides fragilis||NCTC 9343||Grey colonies|
|Clostridium perfringens||NCTC 8237||Grey colonies|
|Escherichia coli||NCTC 12241||Inhibited|
|Staphylococcus aureus||NCTC 12981||Inhibited|
|Proteus mirabilis||NCTC 10975||Inhibited|
Recommended incubation: Incubate anaerobically at 37 ± 1°C for 24-48 hours.