KM0019 – Yeast Extract Glucose Chloramphenicol Agar

//KM0019 – Yeast Extract Glucose Chloramphenicol Agar

KM0019 – Yeast Extract Glucose Chloramphenicol Agar

This is a selective medium for the isolation and enumeration of yeasts and moulds in dairy products. It is in according to a typical formulation of The International Organisation for Standardisation (ISO).
Yeast extract acts as a source of nitrogen, carbon and vitamins in this medium. Glucose is a fermentable carbohydrate. Although the medium has a low pH it is made more selective by the inclusion of chloramphenicol, an antibiotic selective agent.

 

SKU: KM0019 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Candida albicans NCPF 3179 Growth, white colonies
Saccharomyces cerevisiae NCPF 3178 Growth, white colonies
Aspergillus brasiliensis NCPF 2275 Growth, white mycelium, black spores
Escherichia coli NCTC 12241 Inhibited

 

Formula*

g/L
Yeast extract 5.0
Glucose 20.0
Chloramphenicol 0.1
Agar 14.9
Total 40.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 40g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes to completely dissolve the medium. Cool to 42-46°C, mix well and dispense into appropriate sterile containers.