Eosine Methylene Blue Agar

//Eosine Methylene Blue Agar

Eosine Methylene Blue Agar

SKU: KM0028 Category:

Eosin Methylene Blue agar (EMB) is a selective medium primarily for the isolation of coliforms from clinical, food and environmental samples. This is the modified formulation of EMB proposed by Levine with a higher concentration of lactose and the sucrose omitted.
The peptone provides the required carbon, nitrogen and vitamins. Lactose is a fermentable carbohydrate and di-potassium phosphate is a buffer. Eosin Y and methylene blue are indicators. Methylene blue is also a selective agent. During strong acidic conditions, the dyes impart a metallic sheen to certain lactose fermenters, such as Escherichia coli.


Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Growth, blue-Black centered colonies with green metallic sheen
Salmonella typhimurium NCTC 12023 Growth, colourless to amber colonies
Enterococcus faecalis NCTC 12697 Inhibited



Peptone 10.0
Lactose 10.0
Di-potassium phosphate 2.0
Eosin Y 0.4
Methylene blue 0.065
Agar 15.0
Total 37.5
*Adjusted/supplemented as required to meet performance requirements


Suspend 37.5g of the medium in one litre of deionised / purified
water. Allow the medium to soak whilst mixing for 10 minutes. Heat
with frequent agitation and boil for one minute to completely dissolve
the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and
aseptically dispense into appropriate sterile containers.

NB: Store plates in the dark and protect from light