KM0119 – Letheen Agar Modified

//KM0119 – Letheen Agar Modified

KM0119 – Letheen Agar Modified

Letheen agar modified is intended for use in the isolation of microorganisms from cosmetics.
The casein peptone, meat peptone, beef extract and yeast extract act as carbon, nitrogen and vitamin sources in this medium. Glucose is a fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. The sodium bisulfite, lecithin and polysorbate 80 inactivates quaternary ammonium compounds. Polysorabte 80 neutralises phenols, formalin, hexachlorophene, and in combination with the lecithin, ethanol.

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Escherichia coliATCC 8739Growth, cream colonies
Enterococcus faecalisNCTC12697Growth, white colonies
Pseudomonas aeruginosaATCC 9027Growth, cream colonies
Staphylococcus aureusATCC 6538Growth, white colonies

Formula*

FORMULA* g/L
Casein peptone 10
Meat peptone 10
Polysorbate 80 7
Sodium chloride 5
Beef extract 3
Yeast extract 2
Glucose 1
Lecithin 1
Sodium bisulfite 0.1
Agar15
Total 54.1

Preparation

Suspend 54.1g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers