LS0030 – Listeria Oxford Selective Supplement

//LS0030 – Listeria Oxford Selective Supplement

LS0030 – Listeria Oxford Selective Supplement

Listeria Oxford Selective Supplement
E&O Laboratories Ltd Listeria Oxford Selective Supplement (LS0030) is a selective mixture used to supplement Listeria Oxford agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from clinical and food samples.

 

SKU: LS0030 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Dehydrated Medium Colour

PH

Solubility Description

Product Description

Organisms Ref. No Result
Tested as PP0630 (Listeris Oxford Agar)
Listeria monocytogenes NCTC 11994 Growth & Black colonies
Escherichia coli NCTC 12241 Inhibited
Enterococcus faecalis NCTC 12697 Inhibited
Candida albicans NCPF 3179 Inhibited

 

Procedure

To reconstitute the contents of each vial, aseptically add 3ml of Sterile Deionised Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and the resulting solution should be an opaque, yellow colour and free from visible particulate matter. Immediately add the contents of the vial to 500ml of sterile Listeria Oxford agar prepared according to the manufacturer’s instructions and allowed to cool to 45- 50°C.

Mix gently but thoroughly and pour aseptically into sterile Petri dishes. Overnight storage at 2 – 8°C is recommended to allow equilibration of the supplement within the medium.

Formulation

Vial contents:
Colistin sulphate 10.0mg
Amphotericin B 5.0mg
Cefotetan 1.0mg
Phosphomycin 5.0mg
Acriflavine 2.5mg