To reconstitute the contents of each vial, aseptically add 3ml of Sterile Deionised Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and the resulting solution should be an opaque, yellow colour and free from visible particulate matter. Immediately add the contents of the vial to 500ml of sterile Listeria Oxford agar prepared according to the manufacturer’s instructions and allowed to cool to 45- 50°C.
Mix gently but thoroughly and pour aseptically into sterile Petri dishes. Overnight storage at 2 – 8°C is recommended to allow equilibration of the supplement within the medium.