Mueller Hinton Agar Chocolate is used for the isolation and cultivation of fastidious bacteria from clinical specimens. It may also be used for the susceptibility testing of Neisseria gonorrhoeae. Mueller Hinton Agar Chocolate is an enriched, non-selective medium on which fastidious and non-fastidious bacteria, including normal flora, will grow. Therefore, it is recommended to inoculate specimens also onto appropriate selective media. The term “fastidious bacteria” relates to bacteria that do not grow or do not grow well on normally used primary isolation media containing sheep blood.
|Shelf Life (days)|
|Storage Temp (°C)|
Incubate at 37 degrees +/- 1 degree in a 5-10% CO2 and high humidity atmosphere for 36-48 hours.
|Neisseria gonorrhoea||NCTC 8375||Opaque/grey colonies|
|Neisseria gonorrhoea||NCTC 12700||Opaque/grey colonies|
Recommended incubation: For growth promotion, incubate anaerobically at 37 ± 1°C in 5-10% CO2 for 36-48 hours.
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