This is a selective medium for the isolation of clinically significant anaerobes including fastidious organisms. The formulation is complex and includes detoxification agents (starch and sodium hydrogen carbonate), growth enhancing agents (cysteine, arginine, menadione, sodium succinate, glucose and pyrophosphate) and haemin to encourage pigment production in Porphyromonas melaninogenicus. Sodium pyruvate is included to help neutralise hydrogen peroxide. The medium is made selective by the inclusion of nalidixic acid and further enriched by the addition of 7% defibrinated horse blood. Tween 80 is added to encourage the growth of anaerobic streptococci.
|Shelf Life (days)|
|Storage Temp (°C)|
|Bacteroides fragilis||NCTC 9343||Grey colonies|
|Escherichia coli||NCTC 12241||Inhibited|
Recommended incubation: Incubate in anaerobic conditions at 37 ± 1°C for 24-48 hours
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