This is a selective medium for the isolation of Legionella spp used primarily in clinical and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-Cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer and Potassium hydroxide are incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Cefamandole and Polymixin to inhibit most gram positive and gram negative organisms and Cyclohexamide is also included to inhibit yeasts and fungi.
PP0070 – Legionella BMPA Agar
|Shelf Life (days)|
|Storage Temp (°C)|
Plates should be incubated at 35°C ± 2°C in high humidity (90% recommended) for up to 10 days. After 3 days plates should be examined daily.
|Legionella pneumophila||NCTC 11192||Bluish/white translucent colonies|
|Escherichia coli||NCTC 12241||Inhibited|
|Staphylococcus aureus||NCTC 12981||Inhibited|
|Saccharomyces cerevisiae||NCPF 3178||Inhibited|