Slanetz & Bartley Medium

Slanetz & Bartley Medium

Originally intended as a medium for the enumeration of enterococci in water using Membrane Filtration, this medium has become more popular in many other areas such as food bacteriology. The medium contains Tetrazolium Chloride, which is reduced by enterococci to the insoluble red dye Formazan resulting in dark red colonies of enterococci on the agar. It should be noted that this reaction is not exclusive to enterococci and colonies should be confirmed by additional testing e.g. Aesculin hydrolysis.

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Organisms Ref. No Result
Enterococcus faecalis NCTC 12697 Dark red/maroon colonies
Enterococcus faecium ATCC 6057 Dark red/maroon colonies
Escherichia coli NCTC 12241 Inhibited
Staphylococcus aureus NCTC 12981 Inhibited